Human P cadherin ELISA Kit (ab100621)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 2.47 pg/ml - 18000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human P cadherin ELISA Kit
P cadherin キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 20 pg/ml -
検出範囲
2.47 pg/ml - 18000 pg/ml -
添加回収試験
> 100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 129.1 117% - 138% Serum 121.3 111% - 131% Plasma 114.9 105% - 125% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s P cadherin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human P cadherin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human P cadherin coated on a 96-well plate. Standards and samples are pipetted into the wells and P cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human P cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of P cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human P cadherin 2 vials P cadherin Microplate (12 x 8 wells) 1 unit Recombinant Human P cadherin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
組織特異性
Expressed in some normal epithelial tissues and in some carcinoma cell lines. -
関連疾患
Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly. -
配列類似性
Contains 5 cadherin domains. -
細胞内局在
Cell membrane. - Information by UniProt
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別名
- CADH3_HUMAN
- Cadherin 3
- Cadherin 3 precursor
see all -
参照データベース
- Entrez Gene: 1001 Human
- Omim: 114021 Human
- SwissProt: P22223 Human
- Unigene: 191842 Human
画像
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Typical Standard Curve
Representative Standard Curve using ab100621.
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Typical Standard CurveRepresentative Standard Curve using ab100621.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab100621 は論文での使用が確認できていません。