Human NRG1 beta 1 ELISA Kit (ab100614)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 82.3 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human NRG1 beta 1 ELISA Kit -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 50 pg/ml -
検出範囲
82.3 pg/ml - 20000 pg/ml -
添加回収試験
99 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 114.9 102% - 123% Plasma 86.02 79% - 94% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human NRG1 beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NRG1 beta 1 in plasma, and cell culture supernatants. (Collect plasma using EDTA or citrate as an anticoagulant. Heparinized plasma is not recommended. Human NRG1 beta 1 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human NRG1 beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NRG1 beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NRG1 beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human NRG1 beta 1 in normal serum with ab100614, only in serum spiked with Human NRG1 beta 1.
The ab100614 kit recognises the human HRG beta 1 protein (isoform 6) and has not been tested with other isoforms.
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特記事項
Optimization may be required with urine samples
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human NRG1 beta 1 2 vials NRG1 beta 1 Microplate (12 x 8 wells) 1 unit Recombinant Human NRG1 beta 1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. -
組織特異性
Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. -
関連疾患
Note=A chromosomal aberration involving NRG1 produces gamma-heregulin. Translocation t(8;11) with ODZ4. The translocation fuses the 5'-end of ODZ4 to NRG1 (isoform 8). The product of this translocation was first thought to be an alternatively spliced isoform. Gamma-heregulin is a soluble activating ligand for the ERBB2-ERBB3 receptor complex and acts as an autocrine growth factor in a specific breast cancer cell line (MDA-MB-175). Not detected in breast carcinoma samples, including ductal, lobular, medullary, and mucinous histological types, neither in other breast cancer cell lines. -
配列類似性
Belongs to the neuregulin family.
Contains 1 EGF-like domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
発生段階
Detectable at early embryonic ages. Isoform 10 is highly expressed in developing spinal motor neurons and in developing cranial nerve nuclei. Expression is maintained only in both adult motor neurons and dorsal root ganglion neurons. Type IV isoforms are expressed in fetal brain. -
ドメイン
The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
ERBB receptor binding is elicited entirely by the EGF-like domain. -
翻訳後修飾
Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage. -
細胞内局在
Secreted; Cell membrane. Does not seem to be active; Membrane. May possess an internal uncleaved signal sequence; Nucleus. May be nuclear and Secreted. Has a signal peptide. - Information by UniProt
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別名
- Acetylcholine receptor inducing activity
- Acetylcholine receptor-inducing activity
- ARIA
see all -
参照データベース
- Entrez Gene: 3084 Human
- SwissProt: Q02297 Human
- Unigene: 453951 Human
- Unigene: 668810 Human
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab100614 は 6 報の論文で使用されています。
- Chambliss C et al. Elevated neuregulin-1β levels correlate with plasma biomarkers of cerebral injury and high stroke risk in children with sickle cell anemia. Endocr Metab Sci 3:N/A (2021). PubMed: 35935682
- Ferdousi F et al. Microarray analysis of verbenalin-treated human amniotic epithelial cells reveals therapeutic potential for Alzheimer's Disease. Aging (Albany NY) 12:5516-5538 (2020). PubMed: 32224504
- De Iuliis F et al. Circulating neuregulin-1 and galectin-3 can be prognostic markers in breast cancer. Int J Biol Markers 32:e333-e336 (2017). PubMed: 28430337
- Stindt S et al. Hepatitis C Virus Activates a Neuregulin-Driven Circuit to Modify Surface Expression of Growth Factor Receptors of the ErbB Family. PLoS One 11:e0148711 (2016). PubMed: 26886748
- Fedorenko IV et al. BRAF Inhibition Generates a Host-Tumor Niche that Mediates Therapeutic Escape. J Invest Dermatol 135:3115-3124 (2015). PubMed: 26302068
- Cohen JE et al. A bioengineered hydrogel system enables targeted and sustained intramyocardial delivery of neuregulin, activating the cardiomyocyte cell cycle and enhancing ventricular function in a murine model of ischemic cardiomyopathy. Circ Heart Fail 7:619-26 (2014). Sandwich ELISA . PubMed: 24902740