Human Neuro 抗体 Array I (Membrane, 20 Targets) (ab211062)
製品の概要
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製品名
Human Neuro Antibody Array I (Membrane, 20 Targets) -
サンプルの種類
Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate -
種交差性
交差種: Human -
製品の概要
Abcam’s Human Neuro Antibody Array I (ab211062) for use with cell culture media, serum, plasma, cell and tissue lysates and other liquid samples.
Targets: BDNF, beta-NGF, GCSF, GDNF, HB-EGF, IFN gamma, IGF-1, IL-10, IL-1 alpha, IL-1 beta, IL-6, IL-8, MCP-1, MIP-1 alpha, MMP-2, MMP-3, S100B, TGF beta, TNF alpha, VEGF-A.
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特記事項
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about cytokine arrays and other membrane antibody arrays
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Multiplex Protein Detectionmore details
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 2 Membrane 4 Membrane 8 Membrane 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Antibody Arrays 2 units 4 units 8 units Biotinylated Antibody Cocktail 1 vial 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Plastic sheets 1 unit 1 unit 1 unit
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab211062の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Multiplex Protein Detection |
Use at an assay dependent concentration.
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特記事項 |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab211062 は論文での使用が確認できていません。