Human N-Cadherin ELISA Kit (ab254512)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 41 pg/ml
- Range: 156.25 pg/ml - 10000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow, Human
製品の概要
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製品名
Human N-Cadherin ELISA Kit
N Cadherin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 4.4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 9.8% -
サンプルの種類
Cell culture supernatant, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
41 pg/ml -
検出範囲
156.25 pg/ml - 10000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 92 81% - 102% Serum 114 113% - 115% Cell culture extracts 97 84% - 108% Hep Plasma 92 89% - 95% EDTA Plasma 108 106% - 109% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Cow, Human -
製品の概要
Human N-Cadherin ELISA Kit (ab254512) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of N-Cadherin protein in edta plasma, hep plasma, cell culture extracts, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human N-Cadherin with 41 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
N-Cadherin is a member of the calcium-dependent adhesion molecule family of classical cadherins encoded by the CDH2 gene. This transmembrane, homophilic glycoprotein often can be found concentrated at adherens junctions in most cell types. The function of N-Cadherin depends much on the cellular contents such as cancer metastasis, interactions with catenins in neutral tissues, and maintaining the structural integrity of the heart by mediating strong adhesion between cardiomyocytes. This kit is raised as an extracellular fragment of N-Cadherin.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human N-Cadherin Capture Antibody 1 x 600µl 10X Human N-Cadherin Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human N-Cadherin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. -
配列類似性
Contains 5 cadherin domains. -
細胞内局在
Cell membrane. - Information by UniProt
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別名
- CADH2_HUMAN
- Cadherin 2
- Cadherin 2 N cadherin neuronal
see all -
参照データベース
- Entrez Gene: 281062 Cow
- Entrez Gene: 1000 Human
- Omim: 114020 Human
- SwissProt: P19022 Human
- Unigene: 464829 Human
- Unigene: 606106 Human
関連製品
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Alternative Versions
画像
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The N-Cadherin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of N-Cadherin were measured in duplicates, interpolated from the N-Cadherin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and HeLa cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean N-Cadherin concentration was determined to be 17,850 pg/mL in serum, 19,670 pg/mL in plasma (EDTA) and 12,801 pg/mL in plasma (heparin) and 8391 pg/mL in HeLa cell culture supernatant.
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The concentrations of N-Cadherin were measured in duplicate and interpolated from the N-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean N-Cadherin concentration was determined to be 13,895 pg/mL in HeLa cell extract.
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To learn more about the advantages of recombinant antibodies see here.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab254512 は 5 報の論文で使用されています。
- Han YL et al. COL5A2 Inhibits the TGF-β and Wnt/β-Catenin Signaling Pathways to Inhibit the Invasion and Metastasis of Osteosarcoma. Front Oncol 12:813809 (2022). PubMed: 35280775
- Fu J et al. MicroRNA-451a prevents cutaneous squamous cell carcinoma progression via the 3-phosphoinositide-dependent protein kinase-1-mediated PI3K/AKT signaling pathway. Exp Ther Med 21:116 (2021). PubMed: 33335579
- Zhu X et al. Long non-coding RNA TTN antisense RNA 1 facilitates hepatocellular carcinoma progression via regulating miR-139-5p/SPOCK1 axis. Bioengineered 12:578-588 (2021). PubMed: 33517826
- Lei H et al. Baicalein modulates the radiosensitivity of cervical cancer cells in vitro via miR-183 and the JAK2/STAT3 signaling pathway. Adv Clin Exp Med 30:727-736 (2021). PubMed: 34118143
- Cadena M et al. Prostate Cancer Epithelial Mesenchymal Transition by TGF-ß1 exposure monitored with a Photonic Crystal Biosensor. Proc SPIE Int Soc Opt Eng 11251:N/A (2020). PubMed: 32632340