Human MMP9 Matched 抗体 Pair Kit (ab213741)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 15.6 pg/ml - 1000 pg/ml
製品の概要
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製品名
Human MMP9 Matched Antibody Pair Kit
MMP9 キット 製品一覧 -
検出方法
Colorimetric -
アッセイタイプ
ELISA set -
検出感度
3.77 pg/ml -
検出範囲
15.6 pg/ml - 1000 pg/ml -
種交差性
交差種: Human -
製品の概要
Human MMP9 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human MMP9.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 5 x 96 tests Human MMP9 Capture Antibody 1 x 50µg Human MMP9 Detector Antibody 1 x 12.5µg Human MMP9 Lyophilized Protein 1 vial -
研究分野
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機能
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
組織特異性
Produced by normal alveolar macrophages and granulocytes. -
関連疾患
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
配列類似性
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
細胞内局在
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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別名
- 82 kDa matrix metalloproteinase-9
- 92 kDa gelatinase
- 92 kDa type IV collagenase
see all -
参照データベース
- Entrez Gene: 4318 Human
- Omim: 120361 Human
- SwissProt: P14780 Human
- Unigene: 297413 Human
関連製品
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Alternative Versions
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Related Products
画像
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Standard calibration curve. Background subtracted values are graphed. Data provided for demonstration purposes only.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab213741 は論文での使用が確認できていません。