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ab134004 is for simultaneous detection of 7 Human MMPs and 3 TIMPs. Suitable for all sample types.
Targets: MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4
Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
|内容||1 x 4 Membranes||1 x 8 Membranes|
|1,000X HRP-Streptavidin Buffer||1 x 50µl||1 x 50µl|
|20X Wash Buffer I||1 x 10ml||1 x 20ml|
|20X Wash Buffer II||1 x 10ml||1 x 20ml|
|2X Cell Lysis Buffer||1 x 10ml||1 x 16ml|
|8-Well Incubation Tray (with Lid)||1 unit||1 unit|
|Biotin-Conjugated Anti-Cytokines||2 vials||4 vials|
|1X Blocking Buffer||1 x 25ml||2 x 25ml|
|Cytokine Antibody Array Membranes||4 units||8 units|
|Detection Buffer C||1 x 1.5ml||1 x 2.5ml|
|Detection Buffer D||1 x 1.5ml||1 x 2.5ml|
Our Abpromise guarantee covers the use of ab134004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004.
Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.
143B cells (Human osteosarcoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.
Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134004.
Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"