Human MCP2 ELISA Kit (ab223856)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.1 pg/ml
- Range: 1.25 pg/ml - 160 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human MCP2 ELISA Kit
MCP2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Cell Extract 3 3.2% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Cell Extract 3 10.9% -
サンプルの種類
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.1 pg/ml -
検出範囲
1.25 pg/ml - 160 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 132 123% - 146% Cell culture extracts 99 84% - 117% Hep Plasma 113 112% - 117% EDTA Plasma 94 85% - 100% Cit plasma 126 115% - 138% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human MCP2 ELISA Kit (ab223856) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MCP2 with 1.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Monocyte chemoattractant protein 2 (MCP2), known also as C-C motif chemokine 8 (CCL8) is chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. Chemokines and their receptors, in conjunction with adhesion molecules and their ligands, provide cues to enable tissue-specific homing of T cells in the steady state and during inflammation. The processed form MCP2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and MCP2. MCP2 protein can bind heparin.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml 10X Human MCP2 Capture Antibody 1 x 600µl 10X Human MCP2 Detector Antibody 1 x 600µl Human MCP2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 75BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. -
組織特異性
Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
翻訳後修飾
N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Ccl8
- CCL8_HUMAN
- HC14
see all -
参照データベース
- Entrez Gene: 6355 Human
- Omim: 602283 Human
- SwissProt: P80075 Human
- Unigene: 271387 Human
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of MCP2 were measured in duplicates, interpolated from the MCP2 standard curves. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated values are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 19 pg/mL in neat serum, 16 pg/mL in neat plasma (citrate), 18 pg/ml in neat plasma (heparin), and 15 pg/mL in neat plasma (EDTA).
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The concentrations of MCP2 were measured in duplicates, interpolated from the MCP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC treated supernatant 0.08%, PBMC untreated supernatant 0.08%. The interpolated dilution factor corrected values of treated PBMC supernatants are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 80,341 pg/mL in neat treated PBMC supernatant and undetectable in untreated supernatants (not shown).
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of MCP2 were measured in duplicates, interpolated from the MCP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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The concentrations of MCP2 were measured in duplicate and interpolated from the MCP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 37 pg/ml in treated PBMC extract and undetectable in untreated PBMC extract (not shown).
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Interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 21 pg/mL with a range of 12-32 pg/mL in neat serum.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab223856 は論文での使用が確認できていません。