Human MAP1LC3A peptide (ab99367)
Key features and details
- Purity: > 70% HPLC
製品の詳細
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製品名
Human MAP1LC3A peptide
MAP1LC3A タンパク質・ペプチド 製品一覧 -
精製度
> 70 % HPLC.
70 - 90% by HPLC -
アクセッション番号
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Animal free
No -
由来
Synthetic -
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生物種
Human
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特性
Our Abpromise guarantee covers the use of ab99367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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製品の状態
Liquid -
備考
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
関連情報
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別名
- ATG8E
- Autophagy related protein LC3 A
- Autophagy related ubiquitin like modifier LC3 A
see all -
機能
Probably involved in formation of autophagosomal vacuoles (autophagosomes). -
組織特異性
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. -
配列類似性
Belongs to the MAP1 LC3 family. -
翻訳後修飾
The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. -
細胞内局在
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab99367 は論文での使用が確認できていません。
