Human M-CSF ELISA Kit (ab245714)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.8 pg/ml
- Range: 7.8 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Hep Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human M-CSF ELISA Kit
M-CSF キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% plasma 8 6.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% plasma 3 9.9% -
サンプルの種類
Cell culture supernatant, Saliva, Urine, Serum, Hep Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
3.8 pg/ml -
検出範囲
7.8 pg/ml - 500 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 95 87% - 106% Saliva 99 98% - 101% Urine 92 85% - 97% Serum 117 114% - 122% Hep Plasma 110 107% - 113% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human M-CSF ELISA Kit (ab245714) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of M-CSF protein in hep plasma, saliva, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human M-CSF with 3.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human M-CSF Capture Antibody 1 x 600µl 10X Human M-CSF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human M-CSF Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. -
翻訳後修飾
Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated. -
細胞内局在
Cell membrane and Secreted > extracellular space. - Information by UniProt
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別名
- Colony stimulating factor 1
- Colony stimulating factor 1 (macrophage)
- Colony stimulating factor macrophage specific
see all -
参照データベース
- Entrez Gene: 1435 Human
- Omim: 120420 Human
- SwissProt: P09603 Human
- Unigene: 591402 Human
関連製品
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Alternative Versions
画像
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Standard Curve comparison between human M-CSF SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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The M-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2%, plasma (heparin) 2.5%, and PBMC supernatant 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 11.5 ng/mL in serum, 6.7 ng/mL in plasma (heparin), and 2.4 ng/mL in stimulated PBMC supernatant.
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The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 25%, urine 1:200, and A549 cell supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 0.9 ng/mL in saliva, 28.1 ng/mL in urine, and 0.89 ng/mL in A549 cell supernatant.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 27.8 ng/mL with a range of 17.7 - 47.2 ng/mL.
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To learn more about the advantages of recombinant antibodies see here.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245714 は論文での使用が確認できていません。