Human LYVE1 ELISA Kit (ab238263)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4 pg/ml
- Range: 23.44 pg/ml - 1500 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human LYVE1 ELISA Kit
LYVE1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 3.4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 8% -
サンプルの種類
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
4 pg/ml -
検出範囲
23.44 pg/ml - 1500 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Urine 105 101% - 108% Serum 108 103% - 115% Hep Plasma 110 106% - 115% EDTA Plasma 110 105% - 117% Cit plasma 113 107% - 117% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human LYVE1 ELISA Kit (ab238263) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LYVE1 protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LYVE1 with 4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human LYVE1 Capture Antibody 1 x 600µl 10X Human LYVE1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Human LYVE1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
-
機能
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. -
組織特異性
Mainly expressed in endothelial cells lining lymphatic vessels. -
配列類似性
Contains 1 Link domain. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. - Information by UniProt
-
別名
- Cell surface retention sequence-binding protein 1
- CRSBP 1
- CRSBP-1
see all -
参照データベース
- Entrez Gene: 10894 Human
- Omim: 605702 Human
- SwissProt: Q9Y5Y7 Human
- Unigene: 655332 Human
関連製品
-
Alternative Versions
画像
-
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
The LYVE1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
The concentrations of LYVE1 were measured in duplicates, interpolated from the LYVE1 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (EDTA) 1:500, plasma (citrate) 1:500, and plasma (heparin) 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LYVE1 concentration was determined to be 375 ng/mL in plasma (EDTA), 591 ng/mL in plasma (citrate) and 372 ng/mL in plasma (Heparin).
-
The concentrations of LYVE1 were measured in duplicates, interpolated from the LYVE1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:500, urine 1:20. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LYVE1 concentration was determined to be 486 ng/mL in serum and 33 ng/mL in urine.
-
To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab238263 は 1 報の論文で使用されています。
- Ou YC et al. Multimodal Multiplexed Immunoimaging with Nanostars to Detect Multiple Immunomarkers and Monitor Response to Immunotherapies. ACS Nano 14:651-663 (2020). PubMed: 31851488