Human Lysozyme ELISA Kit (LZM) (ab108880)
Key features and details
- Sensitivity: 39 pg/ml
- Range: 0.156 ng/ml - 5 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Other biological fluids, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Lysozyme ELISA Kit (LZM)
Lysozyme キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 4.7% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.5% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Other biological fluids, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 39 pg/ml -
検出範囲
0.156 ng/ml - 5 ng/ml -
添加回収試験
= 98 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human Lysozyme (LZM) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human Lysozyme in plasma, serum, urine, saliva, CSF and cell culture supernatants.
A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lysozyme specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lysozyme captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Lysozyme Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Lysozyme Microplate (12 x 8 well strips) 1 unit Lysozyme Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. -
関連疾患
Amyloidosis 8 -
配列類似性
Belongs to the glycosyl hydrolase 22 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- 1
- 1 4 beta N acetylmuramidase C
- 4-beta-N-acetylmuramidase C
see all -
参照データベース
- Entrez Gene: 4069 Human
- Omim: 153450 Human
- SwissProt: P61626 Human
- Unigene: 524579 Human
画像
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ELISA data
Human lysozyme measured in biological fluids showing quantity (ng) per mL of tested sample. Human serum and plasma diluted 1000-9000 fold. Human saliva diluted 8000-24000 fold. Human urine diluted 10 fold.
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Typical Standard CurveRepresentative Standard Curve using ab108880.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab108880 は 7 報の論文で使用されています。
- Papademas P et al. Investigation of donkey milk bacterial diversity by 16S rDNA high-throughput sequencing on a Cyprus donkey farm. J Dairy Sci 104:167-178 (2021). PubMed: 33162091
- Rana T et al. Linking bacterial enterotoxins and alpha defensin 5 expansion in the Crohn's colitis: A new insight into the etiopathogenetic and differentiation triggers driving colonic inflammatory bowel disease. PLoS One 16:e0246393 (2021). PubMed: 33690604
- Furtado GE et al. Combined Chair-Based Exercises Improve Functional Fitness, Mental Well-Being, Salivary Steroid Balance, and Anti-microbial Activity in Pre-frail Older Women. Front Psychol 12:564490 (2021). PubMed: 33841224
- Makridakis M et al. Multiplexed MRM-based protein quantification of putative prognostic biomarkers for chronic kidney disease progression in plasma. Sci Rep 10:4815 (2020). PubMed: 32179759
- Mao Y et al. Processed human amniotic fluid retains its antibacterial activity. J Transl Med 17:68 (2019). PubMed: 30823930
- Palladino END et al. 2-Chlorofatty acids: lipid mediators of neutrophil extracellular trap formation. J Lipid Res 59:1424-1432 (2018). PubMed: 29739865
- Glorieux G et al. New insights in molecular mechanisms involved in chronic kidney disease using high-resolution plasma proteome analysis. Nephrol Dial Transplant 30:1842-52 (2015). Sandwich ELISA ; Human . PubMed: 26160894