Human LOXL2 ELISA Kit (ab213808)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human LOXL2 ELISA Kit
LOXL2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% 1 16 252pg/ml 11.84 4.7% 2 16 1465pg/ml 114.27 7.8% 3 16 3854pg/ml 254.36 6.6% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% 1 24 263pg/ml 13.93 5.3% 2 24 1490pg/ml 123.67 8.3% 3 24 4228pg/ml 317.1 7.5% -
サンプルの種類
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
156 pg/ml - 10000 pg/ml -
全工程の試験時間
3h 30m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
The Human LOXL2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213808) is designed for the quantitative detection of Human LOXL2 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for LOXL2 has been pre-coated onto 96-well plates. Standards (NSO, Q26-Q774) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LOXL2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LOXL2 amount of sample captured in plate.
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特記事項
Lysyl oxidase homolog 2 is an enzyme that in humans is encoded by the LOXL2 gene. This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue; encoding an extracellular copper-dependent amine oxidase that catalyzes the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control and chemotaxis to each member of the family. LOXL2 can also crosslink collagen type IV and hence influence the sprouting of new blood vessels.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Adhesive Plate Seal 4 units 4 units Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human LOXL2 coated Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti-Human LOXL2 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Human LOXL2 standard 2 vials 2 vials Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
研究分野
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組織特異性
Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate. -
配列類似性
Belongs to the lysyl oxidase family.
Contains 4 SRCR domains. -
翻訳後修飾
The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
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別名
- LOR 2
- LOR2
- LOX L2
see all -
参照データベース
- Entrez Gene: 4017 Human
- Omim: 606663 Human
- SwissProt: Q9Y4K0 Human
- Unigene: 626637 Human
- Unigene: 661130 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab213808 は 1 報の論文で使用されています。
- Bennington J et al. Chemokine Therapy in Cats With Experimental Renal Fibrosis and in a Kidney Disease Pilot Study. Front Vet Sci 8:646087 (2021). PubMed: 33748219