Human Interferon gamma ELISA Kit (IFNG) (ab108863)
Key features and details
- Sensitivity: 0.01 ng/ml
- Range: 0.016 ng/ml - 1 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Interferon gamma ELISA Kit (IFNG)
Interferon gamma キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5.2% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 9.8% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
> 0.01 ng/ml -
検出範囲
0.016 ng/ml - 1 ng/ml -
添加回収試験
101 %
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全工程の試験時間
5h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Interferon gamma (IFNG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interferon gamma levels in cell culture supernatants, serum and plasma.
An Interferon gamma specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Interferon gamma specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Interferon gamma captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Human Interferon gamma Antibody 1 x 150µl Chromogen Substrate 1 x 8ml Interferon gamma Microplate (12 x 8 well strips) 1 unit Interferon gamma Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
研究分野
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機能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
組織特異性
Released primarily from activated T lymphocytes. -
関連疾患
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
配列類似性
Belongs to the type II (or gamma) interferon family. -
翻訳後修飾
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
細胞内局在
Secreted. - Information by UniProt
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別名
- IF 1
- IFG
- IFI
see all -
参照データベース
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab108863 は論文での使用が確認できていません。