Human Interferon Alpha 2 ELISA Kit (ab233622)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 56.04 pg/ml
- Range: 187.5 pg/ml - 12000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Interferon Alpha 2 ELISA Kit
Interferon alpha 2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Spiked serum 3 2.7% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Spiked serum 8 9.3% -
サンプルの種類
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
56.04 pg/ml -
検出範囲
187.5 pg/ml - 12000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 103 99% - 109% Cell culture media 106 100% - 116% Hep Plasma 104 94% - 108% EDTA Plasma 87 80% - 93% Cit plasma 103 102% - 103% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Interferon Alpha 2 ELISA Kit (ab233622) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon Alpha 2 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Interferon Alpha 2 with 56.04 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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試験プラットフォーム
Microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10 x 96 tests 10X Human Interferon Alpha 2 Capture Antibody 1 x 600µl 1 x 6000µl 10X Human Interferon Alpha 2 Detector Antibody 1 x 600µl 1 x 6000µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml Antibody Diluent 5BI 1 x 6ml 10 x 6ml Human Interferon Alpha 2 Lyophilized Purified Protein 2 vials 2 x 10 vials Plate Seals 1 unit 1 x 10 units Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units Stop Solution 1 x 12ml 1 x 120ml TMB Development Solution 1 x 12ml 1 x 120ml -
研究分野
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機能
Produced by macrophages, IFN-alpha have antiviral activities. -
配列類似性
Belongs to the alpha/beta interferon family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Alpha 2a interferon
- IFN
- IFN alpha 2
see all -
参照データベース
- Entrez Gene: 3440 Human
- Omim: 147562 Human
- SwissProt: P01563 Human
- Unigene: 211575 Human
関連製品
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Alternative Versions
画像
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Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Interferon Alpha 2 were measured in duplicates, interpolated from the Interferon Alpha 2 standard curves.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab233622 は論文での使用が確認できていません。