Human Insulin ELISA Kit (ab100578)

Thank you very much for your interest in ab100578. To our knowledge, ab100578 has not been tested in non-human primate. Therefore, I can offer a discount off a future purchase if you buy ab100578 now, test it in non-human primate and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free kit of the same value. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab100578 in non-human primate. I will then send a discount code. This code must be issued before purchasing ab100578 so please wait for my reply before ordering. 2. Purchase ab100578 either by phone, fax, or online (www.abcam.com). 3. Test it in non-human primate. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any kit ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab100578 turns out to be unsuitable for non-human primate you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

I am happy to confirm that Heparin, EDTA and Citrate treated plasma samples were all tested with this kit and are all suitable. This is covered by our AbPromise guarantee.

We do not have data for camel samples tested with the human insulin ELISA, ab100578. However, insulin is well-conserved between the two species, so it is possible the ELISA will detect camel insulin.

To check the similarity between the two species, I used the camel UniProt database entry at the following link:

P01320 (INS_CAMDR) http://www.uniprot.org/uniprot/P01320

The pig insulin ELISA ab156515 will be a slightly better choice, since camel insulin is more similar to pig insulin than human.

Click here (or use the following: https://www.abcam.com/Insulin-Pig-ELISA-Kit-ab156515.html).

Here are the percentage identity results of the alignments of camel insulin precursor with human and pig insulin.

93% w/ human insulin B chain http://www.uniprot.org/blast/?about=P01308[25-54]
90% w/ human insulin Achain http://www.uniprot.org/blast/?about=P01308[90-110]

97% w/ pig insulin B chain http://www.uniprot.org/blast/?about=P01315[25-54]
90% w/ pig insulin A chain http://www.uniprot.org/blast/?about=P01308[90-110]

Thanks for your enquiry.

The kit can be used within 1 year if the entire kit is stored unopened at -20 degrees C. After opening, please refer to the protocol booklet for component-specific storage instructions.

The general sample information for the Insulin Human ELISA Kit (ab100578) is a suggested dilution of normal serum/plasma: 2-10 fold (page 10 of the Protocol Booklet).
We however recommend testing different dilutions such as 1:2, 1:5, 1:10 to determine which one is the best for your particular sample.
Once the sample has been diluted, 100ul of sample should be added into appropriate wells and incubate for 2.5 hours at room temperature or over night at 4C with gentle shaking (remember to cover well the plate). For the following steps please follow the kit protocol at page 11 of the Protocol Booklet.

The antibodies used in this kit will recognize Insulin and Proinsulin. From the information which I have been able to gather on SwissProt regarding insulin (http://www.uniprot.org/uniprot/P01308) the insulin precuror is composed of a signal peptide, chains A, B and a C peptide. It seems that insulin is the heterodimer of chain A and chain B and that proinsulin contains chain A, B and peptide C. While I cannot be sure of whether the proinsulin is non functional it seems likely that this form could be compared to the functional heterodimer. If this is incorrect please let me know. Assuming that this is correct, then ab100578 will recognize both forms. I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

Les anticorps utilisés dans le kit ELISA ab100578 sont les anticorps ab8304 comme anticorps de capture et ab20756 comme anticorps de détection. Ces deux anticorps sont testés et garantis chez le rat. Le kit lui-même n’a pas été testé chez le rat mais il y’a des chances que cela fonctionne. Si vous souhaitez le tester, je peux vous faire une offre de test.