Human IL1R2 ELISA Kit (ab269548)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24.96 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL1R2 ELISA Kit
IL-1R-2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 3 1.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 8 2.3% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
24.96 pg/ml -
検出範囲
62.5 pg/ml - 4000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Urine 95 93% - 99% Serum 110 106% - 116% Hep Plasma 110 103% - 118% EDTA Plasma 110 105% - 118% Cit plasma 107 104% - 113% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human IL1R2 ELISA Kit (ab269548) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL1R2 protein in edta plasma, serum, urine, cit plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL1R2 with 24.96 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
IL1R2, interleukin 1 receptor, type II, is a key protein involved in the negative regulation of inflammatory and immune signaling. IL1R2 inhibits interleukins 1a and 1b and IL-1RAcp by binding to them without propagating a signaling cascade. Given its pivotal role in cell signaling, IL1R2 is currently under investigation for its role in a host of inflammatory and autoimmune-related pathologies. The antibodies for this kit were raised against a recombinant protein fragment of the mature human protein. This protein does not share a high sequence identity with mouse or rat and does not react. However, this kit does cross-react with monkey serum.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human IL1R2 Capture Antibody 1 x 600µl 10X Human IL1R2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IL1R2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1ra). -
配列類似性
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. -
細胞内局在
Membrane. - Information by UniProt
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別名
- IL1 Receptor II
- Antigen CDw121b
- CD121
see all -
参照データベース
- Entrez Gene: 7850 Human
- Omim: 147811 Human
- SwissProt: P27930 Human
- Unigene: 25333 Human
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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The IL1R2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of IL1R2 were measured in duplicates, interpolated from the IL1R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:20, plasma (citrate) 1:20, plasma (EDTA) 1:20, and plasma (heparin) 1:20. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R2 concentration was determined to be 40.50 ng/mL in serum, 24.84 ng/mL in plasma (citrate), 30.33 ng/mL in plasma (EDTA), and 31.03 ng/mL in plasma (heparin).
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The concentrations of IL1R2 were measured in duplicates, interpolated from the IL1R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R2 concentration was determined to be 2.87 ng/mL in urine.
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The concentrations of IL1R2 were measured in duplicates, interpolated from the IL1R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC cell culture supernatant 100% and unstimulated PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R2 concentration was determined to be 226 pg/mL in stimulated PBMC cell culture supernatant and was undetectable in unstimulated PBMC cell culture supernatant.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R2 concentration was determined to be 43 ng/mL with a range of 31 – 77 ng/mL.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab269548 は論文での使用が確認できていません。