Human IL-6 ELISPOT 抗体 Pair (ab46600)
Key features and details
- Assay type: Sandwich (quantitative)
- Sample type: Suspension cells
- Reacts with: Human
製品の概要
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製品名
Human IL-6 ELISPOT Antibody Pair
IL-6 キット 製品一覧 -
サンプルの種類
Suspension cells -
アッセイタイプ
Sandwich (quantitative) -
種交差性
交差種: Human -
製品の概要
Intended use
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. ELISPOT assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
The ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Principle of the method
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes natural human IL6.
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アプリケーション
適用あり: ELISpotmore details
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 10 x 96 tests Biotinylated detection antibody, clone B-E4: 2 vials Capture antibody, clone B-E8: 2 vials of 0.50 ml. 2 x 500µl -
研究分野
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機能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
関連疾患
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
配列類似性
Belongs to the IL-6 superfamily. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
参照データベース
- Entrez Gene: 3569 Human
- Omim: 147620 Human
- SwissProt: P05231 Human
- Unigene: 654458 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46600の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISpot |
Use at an assay dependent dilution.
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特記事項 |
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ELISpot
Use at an assay dependent dilution. |
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab46600 は論文での使用が確認できていません。