製品の概要

  • 製品名

    Human IL-5 ELISA Kit
    IL-5 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5 8.6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 5.4%
  • サンプルの種類

    Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    3.3 pg/ml
  • 検出範囲

    4.7 pg/ml - 300 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 105 104% - 107%
    Urine 81 80% - 81%
    Serum 96 93% - 100%
    Hep Plasma 99 97% - 100%
    EDTA Plasma 94 93% - 96%
    Cit plasma 93 90% - 96%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Human
    非交差種: Cow
  • 製品の概要

    IL-5 in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-5 protein in human serum, plasma, cell culture supernatant and urine.


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • 特記事項

    Interleukin 5 (IL-5) is a small, 113 amino acid cytokine produced by T cells involved in the differentiation of B-cells to immunoglobulin secreting cells. IL-5 is a key mediator in the survival and effector functions of mature eosinophils. Due to its effects on eosinophils, IL-5 is implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab215536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between human IL-5 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-5 were measured in duplicates, interpolated from the IL-5 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 149 pg/mL in serum, 131 pg/mL in plasma (citrate), 165 pg/mL in plasma (heparin), 142 pg/mL in plasma (EDTA) and 133 pg/mL in urine.

  • Conditioned media was harvested after 48 hours. IL-5 was measured in 100% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-5 were measured in duplicate, interpolated from the IL-5 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 226.9 pg/mL in PHA stimulated PBMC supernatant and 1.94 pg/mL in unstimulated PBMC supernatant.

プロトコール

参考文献

This product has been referenced in:

  • Yamamoto M  et al. Stage classification of IgG4-related dacryoadenitis and sialadenitis by the serum cytokine environment. Mod Rheumatol N/A:1-5 (2018). Read more (PubMed: 29385874) »
See 1 Publication for this product

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