製品の概要

  • 製品名
    Human IL-5 ELISA Kit
    IL-5 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5 8.6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 5.4%
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    3.3 pg/ml
  • 検出範囲
    4.7 pg/ml - 300 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 105 104% - 107%
    Urine 81 80% - 81%
    Serum 96 93% - 100%
    Heparin Plasma 99 97% - 100%
    EDTA Plasma 94 93% - 96%
    Citrate Plasma 93 90% - 96%

  • 全工程の試験時間
    1h 30m
  • ステップ
    One step assay
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat, Cow
  • 製品の概要

    IL-5 in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-5 protein in human serum, plasma, cell culture supernatant and urine.


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • 特記事項

    Interleukin 5 (IL-5) is a small, 113 amino acid cytokine produced by T cells involved in the differentiation of B-cells to immunoglobulin secreting cells. IL-5 is a key mediator in the survival and effector functions of mature eosinophils. Due to its effects on eosinophils, IL-5 is implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Human IL-5 Capture Antibody 1 x 600µl
    10X Human IL-5 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI 1 x 6ml
    Human IL-5 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Pre-Coated 96-Well Microplate 1 unit
    Sample Diluent NS 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • 研究分野
  • 機能
    Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • 配列類似性
    Belongs to the IL-5 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • B-cell differentiation factor I
    • Colony stimulating factor
    • EDF
    • Eosinophil differentiation factor
    • IL-5
    • IL5
    • IL5_HUMAN
    • Interleukin 5
    • Interleukin 5 (colony stimulating factor, eosinophil)
    • Interleukin-5
    • T Cell Replacing Factor
    • T-cell replacing factor
    • TRF
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab215536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Standard curve comparison between human IL-5 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.
  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-5 were measured in duplicates, interpolated from the IL-5 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 149 pg/mL in serum, 131 pg/mL in plasma (citrate), 165 pg/mL in plasma (heparin), 142 pg/mL in plasma (EDTA) and 133 pg/mL in urine.

  • Conditioned media was harvested after 48 hours. IL-5 was measured in 100% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-5 were measured in duplicate, interpolated from the IL-5 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 226.9 pg/mL in PHA stimulated PBMC supernatant and 1.94 pg/mL in unstimulated PBMC supernatant.

プロトコール

参考文献

This product has been referenced in:
  • Yamamoto M  et al. Stage classification of IgG4-related dacryoadenitis and sialadenitis by the serum cytokine environment. Mod Rheumatol N/A:1-5 (2018). Read more (PubMed: 29385874) »
See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab215536.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録