Human IL-4 Receptor ELISA Kit (ab46022)
Key features and details
- Sensitivity: 11.5 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-4 Receptor ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Sample A 8 563.7pg/ml 17.8 3.1% Sample B 8 140.6pg/ml 9.6 6.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Sample A 8 530.5pg/ml 35.1 6.6% Sample B 8 106.5pg/ml 7.58 7.1% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 11.5 pg/ml -
検出範囲
31.25 pg/ml - 1000 pg/ml -
全工程の試験時間
1h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human IL-4 Receptor (IL-4R) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble IL-4 receptor molecule (CD124) in Human serum, plasma, buffered solutions or cell culture medium.
A monoclonal antibody specific for IL-4R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4R concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4R are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4R present in the samples.
This kit will recognize both endogenous and recombinant Human IL-4R.
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特記事項
The standard is affinity purified.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-IL-4R Red 1 x 0.4ml 2 x 0.4ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml IL-4R Microplate (12 x 8 well strips) 1 unit 2 units IL-4R Standard (Lyophilized) Orange 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
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関連性
IL-4R binds interleukin 4 and interleukin 13 to regulate IgE production. It also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the IL-4R can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Two transcript variants encoding different isoforms, a membrane-bound and a soluble form, have been found for this gene. -
細胞内局在
Cell membrane; single-pass type I membrane protein. Isoform 2: Secreted protein -
別名
- IL 4R alpha
- IL4R
- IL4RA
see all -
参照データベース
- Entrez Gene: 3566 Human
- SwissProt: P24394 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab46022 は 1 報の論文で使用されています。
- Xu G et al. Endoscopic removal of nucleus pulposus of intervertebral disc on lumbar intervertebral disc protrusion and the influence on inflammatory factors and immune function. Exp Ther Med 19:301-307 (2020). PubMed: 31853303