製品の概要

  • 製品名

    Human IL-22 ELISA Kit
    IL-22 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 8 4.6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 2.6%
  • サンプルの種類

    Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    2.61 pg/ml
  • 検出範囲

    13.12 pg/ml - 840 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 101 98% - 104%
    Urine 107 101% - 111%
    Serum 102 100% - 104%
    Hep Plasma 102 99% - 104%
    EDTA Plasma 102 100% - 105%
    Cit plasma 93 91% - 97%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Human
    非交差種: Cow
  • 製品の概要

    IL-22 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-22 protein in human serum, plasma, cell culture supernatant and urine. (UniprotID: Q9GZX6)


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples in Sample Diluent NS: 2.61 pg/mL


    Samples in Sample Diluent 50BS: 4.71 pg/mL

  • 特記事項

    Interleukin 22 (IL-22) is a cytokine thought to be involved in the response to inflammation through the Jak/STAT and MAPK signaling pathways. IL-22 is produced by NK cells and Th1-type T cells and can stimulate the induction of pro-inflammatory cytokines in human keratinocytes.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Human IL-22 Capture Antibody 1 x 600µl
    10X Human IL-22 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human IL-22 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Sample Diluent 50BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • 研究分野

  • 機能

    Cytokine that contributes to the inflammatory response in vivo.
  • 配列類似性

    Belongs to the IL-10 family.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • Cytokine Zcyto18
    • IL 10 related T cell derived inducible factor
    • IL 21
    • IL 22
    • IL D110
    • IL TIF
    • IL-10-related T-cell-derived-inducible factor
    • IL-22
    • IL-TIF
    • IL21
    • Il22
    • IL22_HUMAN
    • ILD110
    • ILTIF
    • Interleukin 10 related T cell derived inducible factor
    • interleukin 21
    • Interleukin 22
    • Interleukin-22
    • MGC79382
    • MGC79384
    • TIFa
    • TIFIL 23
    • TIFIL23
    • UNQ3099/PRO10096
    • zcyto18
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab216170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-22 were measured in duplicates, interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean spiked IL-22 concentration was determined to be 1015 pg/mL in PBMC supernatant.

  • The concentrations of IL-22 were measured in duplicates, interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, plasma (EDTA) 50% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean spiked IL-22 concentration was determined to be 1174 pg/mL in serum, 1099 pg/mL in plasma (citrate), 1197 pg/mL in plasma (heparin), 1189 pg/mL in plasma (EDTA) and 1,322 pg/mL in urine.

  • Conditioned media was harvested after 48 hours. IL-22 was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-22 were measured in duplicate, interpolated from the IL-22 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-22 concentration was determined to be 316 pg/mL in PHA stimulated PBMC supernatant and 93 pg/mL in unstimulated supernatant.

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参考文献

ab216170 has not yet been referenced specifically in any publications.

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