Human IL-2 ELISA Kit (ab270883)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 32.1 pg/ml
- Range: 39 pg/ml - 2500 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-2 ELISA Kit
IL-2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Supernatant 8 3.2% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Supernatant 4 8.7% -
サンプルの種類
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
32.1 pg/ml -
検出範囲
39 pg/ml - 2500 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 108 100% - 120% Serum 86 83% - 88% Hep Plasma 82 80% - 85% EDTA Plasma 89 86% - 93% Cit plasma 87 84% - 91% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human IL-2 ELISA kit (ab270883) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-2 protein in human serum, plasmas and cell and tissue supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human IL-2 with 32.1 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY: This kit recognizes both native and recombinant human IL-2 protein in stimulated serum, plasmas, and cell culture supernatant.
Urine, milk, saliva, and cell and tissue extract samples have not been tested with this kit.
CROSS REACTIVITY: Recombinant human IL-2R alpha was prepared at 5,000 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE: Recombinant human IL-2R alpha was prepared at 5,000 pg/mL and tested for interference. No interference with was observed.
SPECIES REACTIVITY: This kit recognizes human IL-2 protein.
No signal was observed in 50% serum samples from the following species: Mouse, Rat, Cow.
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特記事項
IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical polypeptide, synthesized as a 153 amino acid (aa) precursor with a 20 aa signal peptide and a 133 aa mature chain. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, Natural Killer cells (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. IL-2 modulates the expression of receptors for other cytokines and transcription factors, therefore regulating cytokine cascades that correlate with each of the Th differentiation states. IL-2 is both an immune stimulator and immune suppressor cytokine, which efficiently controls the immune system to deal with autoimmunity and adaptive immune response.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human IL-2 Capture Antibody 1 x 600µl 10X Human IL-2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IL-2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
関連疾患
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
配列類似性
Belongs to the IL-2 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Aldesleukin
- IL 2
- IL-2
see all -
参照データベース
- Entrez Gene: 3558 Human
- Omim: 147680 Human
- SwissProt: P60568 Human
- Unigene: 89679 Human
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Standard Curve comparison between the original Human IL-2 SimpleStep ELISA kit (ab174444) and current Human IL-2 SimpleStep ELISA kit (ab270883).
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The IL-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IL-2 standard curve in Sample Diluent NS. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IL-2 standard curve in Sample Diluent 50BP. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IL-2 standard curve in Sample Diluent 50BP. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of IL-2 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMCs stimulated with 1.5% PHAM for 48 hours (25%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 5,330 pg/mL in stimulated PBMCs.
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The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.
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Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Recombinant IL-2 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent 50BP.
50% pooled serum samples from eight healthy donors was measured in duplicate. All values were below the detectable range of the assay.
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Recombinant IL-2 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
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Native IL-2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in NS.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab270883 は 1 報の論文で使用されています。
- Hudec M et al. Enhanced Extracellular Transfer of HLA-DQ Activates CD3+ Lymphocytes towards Compromised Treg Induction in Celiac Disease. Int J Mol Sci 23:N/A (2022). PubMed: 35682780