製品の概要

  • 製品名

    Human IL-2 ELISA Kit
    IL-2 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 6 6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 24 6%
  • サンプルの種類

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    9 pg/ml
  • 検出範囲

    15.63 pg/ml - 2000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 95 93% - 97%
    Cell culture media 94 83% - 109%
    Heparin Plasma 91 82% - 95%
    EDTA Plasma 100 95% - 105%
    Citrate Plasma 83 77% - 98%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Human
    非交差種: Mouse
  • 製品の概要

    Abcam’s Human IL-2 (Interleukin 2) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit (ab174444) is designed for the quantitative measurement of IL-2 protein in Human cell culture supernatant, plasma and serum samples.


    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm

  • 特記事項

    IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical polypeptide, synthesized as a 153 amino acid (aa) precursor with a 20 aa signal peptide and a 133 aa mature chain. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, Natural Killer cells (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. In particular, IL-2 modulates the expression of receptors for other cytokines and transcription factors, therefore regulating cytokine cascades that correlate with each of the Th differentiation states.

    Complete deficiency of IL-2 has been implicated in severe combined immunodeficiency, whereas reduction of the IL-2 correlates with reduced function of CD4+CD25+ regulatory T cells and destabilization of immune homeostasis leading to autoimmune disease. Increased expression of IL-2 has also been implicated in inflammatory conditions such as inflammatory bowel disease and chronic liver diseases. IL-2 therefore is both a immune stimulator and immune suppressor cytokine which efficiently controls the immune system to deal with autoimmunity and adaptive immune response.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab174444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example IL-2 standard curve in sample diluents NS.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2mM L-glutamine, 100U/mL penicillin, and 100 µg/mL streptomycin. Cells were cultured for 2 days at 37⁰C in the presence or absence of PHA. The concentrations of IL-2 were interpolated from the calibration curve and corrected for sample dilution. The mean IL-2 concentration was determined to be 16 pg/mL in unstimulated PBMC supernatants and 11,460 pg/mL in stimulated PBMC supernatants.

プロトコール

参考文献

This product has been referenced in:

  • Jia Y  et al. Overexpression of CD59 inhibits apoptosis of T-acute lymphoblastic leukemia via AKT/Notch1 signaling pathway. Cancer Cell Int 19:9 (2019). Read more (PubMed: 30636930) »
  • De R  et al. Comparative study of bone marrow and blood plasma levels of IL-2 in aplastic anaemia and their relationship with disease severity. Hematology 24:84-88 (2019). Read more (PubMed: 30139310) »
See all 5 Publications for this product

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