Human IL-18BP ELISA Kit (ab100559)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 24.69 pg/ml - 18000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-18BP ELISA Kit
IL-18BP キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 20 pg/ml -
検出範囲
24.69 pg/ml - 18000 pg/ml -
添加回収試験
91 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 106.7 96% - 121% Serum 83.5 74% - 97% Plasma 82.3 72% - 96% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-18BP (Interleukin-18 Binding Protein) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18BP in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-18BP coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-18BP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 BP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18BP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-18BP 2 vials IL-18BP Microplate (12 x 8 wells) 1 unit Recombinant Human IL-18BP Standard (lyophilized) 2 units Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. -
組織特異性
Strongly expressed in heart, lung, placenta and spleen. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
細胞内局在
Secreted. - Information by UniProt
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別名
- I18BP_HUMAN
- IL-18BP
- IL18 binding protein
see all -
参照データベース
- Entrez Gene: 10068 Human
- Omim: 604113 Human
- SwissProt: O95998 Human
- Unigene: 591967 Human
画像
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Typical standard curve
Representative standard curve using ab100559
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Typical standard curveRepresentative standard curve using ab100559
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab100559 は 3 報の論文で使用されています。
- Nawaz SS et al. Potential role of serum fetuin-A in relation with pro-inflammatory, chemokine and adhesion molecules in diabetic kidney disease: a case-control study. Mol Biol Rep 46:1239-1246 (2019). PubMed: 30632070
- Menale C et al. Bisphenol A effects on gene expression in adipocytes from children: association with metabolic disorders. J Mol Endocrinol 54:289-303 (2015). PubMed: 25878060
- Uzan B et al. Interleukin-18 produced by bone marrow-derived stromal cells supports T-cell acute leukaemia progression. EMBO Mol Med 6:821-34 (2014). PubMed: 24778454