製品の概要

  • 製品名

    Human IL-17E ELISA Kit (IL-25)
    IL-25 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    1 16 621pg/ml 24.2 3.9%
    2 16 1969pg/ml 90.6 4.6%
    3 16 2795pg/ml 150.9 5.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    1 24 730pg/ml 30.7 4.2%
    2 24 2103pg/ml 119.9 5.7%
    3 24 3065pg/ml 208.4 6.8%
  • サンプルの種類

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    < 10 pg/ml
  • 検出範囲

    62.5 pg/ml - 4000 pg/ml
  • 全工程の試験時間

    3h 30m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Human
  • 製品の概要

    The Human IL-17E Enzyme-Linked Immunosorbent Assay (ELISA) kit (IL-25) (ab213793) is designed for the quantitative detection of Human IL-17E in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). 


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IL-17E has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: Y33-G177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17E is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-17E amount of sample captured in plate.

  • 特記事項

    Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-κB activation, and stimulate the production of IL8. What’s more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human IL-17E coated Microplate (12 x 8 wells)  1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human IL-17E antibody 1 x 130µl
    Lyophilized recombinant Human IL-17E standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • 研究分野

  • 機能

    Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
  • 組織特異性

    Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
  • 配列類似性

    Belongs to the IL-17 family.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • IL 17E
    • IL 25
    • IL-17E
    • IL-25
    • IL17e
    • IL25
    • IL25_HUMAN
    • Interleukin 17E
    • Interleukin 25
    • Interleukin-17E
    • Interleukin-25
    • Interleukin17E
    • Interleukin25
    • OTTHUMP00000027946
    • OTTHUMP00000246238
    • UNQ3120/PRO10272
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab213793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Human IL-17E ELISA Kit (IL-25) (ab213793) Standard Curve

プロトコール

参考文献

ab213793 has not yet been referenced specifically in any publications.

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