製品の概要

  • 製品名

    Human IL-17A ELISA Kit
    IL-17A キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3 10.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 5 6.8%
  • サンプルの種類

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    1.1 pg/ml
  • 検出範囲

    1.56 pg/ml - 100 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 97 97% - 98%
    Cell culture media 92 82% - 108%
    Hep Plasma 96 86% - 115%
    EDTA Plasma 106 104% - 110%
    Cit plasma 101 94% - 108%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Human
  • 製品の概要

    Human IL-17A SimpleStep ELISA kit (ab176111) has been re-developed with new capture and detectoe antibodies.This new kit has the same name but a different product number (ab216167). We have identified new recombinant monoclonal antibodies to use in the SimpleStep ELISA platform that provide a higher sensitivity when quantifying IL-17A in human serum, plasma and cell culture supernatants.


     IL-17A in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-17A protein in human serum, plasma, and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • 特記事項

    IL-17A is a proinflammatory cytokine that is secreted by activated T cells. It is a disulfide-linked homodimer with both glycosylated and nonglycosylated forms. IL-17A induces stromal cells to produce proinflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Human IL-17A Capture Antibody 1 x 600µl
    10X Human IL-17A Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human IL-17A Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • 研究分野

  • 機能

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • 組織特異性

    Restricted to activated memory T-cells.
  • 配列類似性

    Belongs to the IL-17 family.
  • 翻訳後修飾

    Found both in glycosylated and nonglycosylated forms.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • CTLA 8
    • CTLA-8
    • CTLA8
    • cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8)
    • interleukin 17A
    • Interleukin-17A
    • interleukin17
    • Interleukin17A
    • OTTHUMP00000016597
    • OTTMUSP00000046003
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab216167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between human IL-17A SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 13-fold increase in sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-17A were measured in duplicates, interpolated from the IL-17A standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC supernatant 10%, and unstimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17A concentration was determined to be 542 pg/mL in neat PHA-M stimulated PBMC supernatant and 43.6 pg/mL in neat unstimulated PBMC supernatant.

  • The concentrations of IL-17A were measured in duplicates, interpolated from the IL-17A standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 46 hours. The concentrations of IL-17A were measured in supernatant samples in 5 different dilutions in duplicates and interpolated from the IL-17 A standard curve. The interpolated values are plotted (mean +/- SD, n=5). The mean IL-17 A concentration was determined to be 542 pg/mL in neat PHA-M stimulated PBMC cell supernatant, 43.6 pg/mL in neat unstimulated supernatants and undetectable in neat media (not shown).

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参考文献

ab216167 has not yet been referenced specifically in any publications.

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