製品の概要

  • 製品名

    Human IL-12 ELISA Kit High Sensitivity
    IL-12 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 16.1pg/ml 1.25 7.8%
    Sample B 8 6.1pg/ml 0.4 6.6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 20 16pg/ml 1.2 7.9%
    Sample B 20 6pg/ml 0.6 9.5%
  • サンプルの種類

    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    < 0.75 pg/ml
  • 検出範囲

    0.78 pg/ml - 25 pg/ml
  • 添加回収試験

    89 %

  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Human
  • 製品の概要

    Human IL-12 ELISA kit High Sensitivity is designed for the quantitative measurement of IL-12 in Human serum, plasma, buffered solutions and cell culture medium.


    A monoclonal antibody specific for IL-12 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 present in the samples. 


    This kit will recognize both endogenous and recombinant Human IL-12.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab46035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab46035

プロトコール

参考文献

This product has been referenced in:

  • Pham PV  et al. Targeting breast cancer stem cells by dendritic cell vaccination in humanized mice with breast tumor: preliminary results. Onco Targets Ther 9:4441-51 (2016). Read more (PubMed: 27499638) »
  • Pham PV  et al. A simple in vitro method for evaluating dendritic cell-based vaccinations. Onco Targets Ther 7:1455-64 (2014). Sandwich ELISA . Read more (PubMed: 25170272) »
See all 2 Publications for this product

レビューと Q&A

Answer

1)

The kit is suitable for tissue homogenates.
2) The suggested homogenate buffer containing 0.5% Tween20, EDTA and Tris is appropriate and compatible with the kit.

Read More

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