Human IL-11 ELISA Kit (ab100551)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 3.29 pg/ml - 800 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-11 ELISA Kit
IL-11 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 3 pg/ml -
検出範囲
3.29 pg/ml - 800 pg/ml -
添加回収試験
98 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 101 89% - 108% Serum 97.89 85% - 104% Plasma 95.5 83% - 103% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-11 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-11 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-11 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human IL-11 ELISA Kit (ab189569) from our SimpleStep ELISA® range.
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特記事項
Optimization may be required with urine samples.
The standard is affinity purified.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 160X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-11 2 vials IL-11 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-11 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. -
配列類似性
Belongs to the IL-6 superfamily. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Adipogenesis inhibitory factor
- AGIF
- IL 11
see all -
参照データベース
- Entrez Gene: 3589 Human
- Omim: 147681 Human
- SwissProt: P20809 Human
- Unigene: 467304 Human
関連製品
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SimpleStep ELISA kits
画像
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Typical standard curve
Representative standard curve using ab100551
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Typical standard curveRepresentative standard curve using ab100551
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab100551 は 1 報の論文で使用されています。
- Sabzevary-Ghahfarokhi M et al. The expression analysis of Fra-1 gene and IL-11 protein in Iranian patients with ulcerative colitis. BMC Immunol 19:17 (2018). PubMed: 29914371