Anti-Human IgM 抗体 [RM121] (ab193159)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM121] to Human IgM
- Suitable for: Sandwich ELISA, ELISA
- Reacts with: Human, Recombinant fragment
製品の概要
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製品名
Anti-Human IgM antibody [RM121]
Human IgM 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [RM121] to Human IgM -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISA, ELISAmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Full length protein corresponding to Human IgM. Human IgM
Database link: P01871
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Protein A affinity purified from an animal origin–free culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
RM121 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab193159の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use at an assay dependent concentration.
Use: 50ng/well – 200ng/well (for Capture); |
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. Use: 50ng/well – 200ng/well (for Capture); |
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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関連性
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. -
細胞内局在
Cell Membrane and Secreted -
参照データベース
- Entrez Gene: 3507 Human
- Omim: 147020 Human
- SwissProt: P01871 Human
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別名
- Immunoglobin heavy chain constant region mu antibody
- AGM1 antibody
- Constant region of heavy chain of IgM antibody
see all
画像
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A titer ELISA using ab193159. The plate was coated with different amounts of human IgM. A serial dilution of ab193159 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Sandwich ELISA using ab193159 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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ELISA of human immunoglobulins shows ab193159 only reacted to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL of ab193159 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Sandwich ELISA using ab193159 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab193159 は論文での使用が確認できていません。