Key features and details
- Mouse monoclonal [4A11] to Human IgG Fab fragment
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2b
製品名Anti-Human IgG Fab fragment antibody [4A11]
製品の詳細Mouse monoclonal [4A11] to Human IgG Fab fragment
特異性This antibody reacts with Fab fragment of human IgG. Specificity was confirmed by western blotting analysis of purified Fab fragment, of human IgG, under reducing conditions.
アプリケーション適用あり: Flow Cyt, WBmore details
Full length native protein (purified) (Human).
- WB: Normal human immunoglobulins. Flow Cyt: Human peripheral blood cells.
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.097% Sodium azide
Concentration information loading...
特記事項（精製）Purified by precipitation and chromatography
Our Abpromise guarantee covers the use of ab771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|WB||Use at an assay dependent concentration.|
関連性Human IgG consists of four subclasses (1-4) that can be recognised by antigen differences in their heavy chains. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. The Fab fragment (fragment antigen binding) is the antigen region of the IgG molecule. It is composed of one constant and one variable domain of each of the heavy and the light chain.
Flow Cytometry analysis of human peripheral blood cells labeling Human IgG Fab fragment with Anti-Human IgG Fab fragment antibody [4A11] (ab771).
All lanes : Anti-Human IgG Fab fragment antibody [4A11] (ab771)
Lane 1 : Normal human immunoglobulins
Lane 2 : Normal bovine immunoglobulins
Performed under reducing conditions.
Surface staining of human peripheral blood cells with ab771. An APC-conjugated goat anti-mouse IgG was used as the secondary antibody.
ab771 は 2 報の論文で使用されています。
- Lau YF et al. An adjuvant for the induction of potent, protective humoral responses to an H5N1 influenza virus vaccine with antigen-sparing effect in mice. J Virol 84:8639-49 (2010). ELISA . PubMed: 20538850
- Drexler SK et al. SIGIRR/TIR-8 is an inhibitor of Toll-like receptor signaling in primary human cells and regulates inflammation in models of rheumatoid arthritis. Arthritis Rheum 62:2249-61 (2010). Flow Cyt ; Human . PubMed: 20506350