Anti-Human IgG Fab fragment 抗体 [4A11] (ab771)

Submit a review Q&A (8)References (2)

Key features and details

  • Mouse monoclonal [4A11] to Human IgG Fab fragment
  • Suitable for: Flow Cyt, ELISA, WB
  • Reacts with: Human
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-Human IgG Fab fragment antibody [4A11]

  • 製品の詳細

    Mouse monoclonal [4A11] to Human IgG Fab fragment

  • 由来種

    Mouse

  • 特異性

    This antibody reacts with Fab fragment of human IgG. Specificity was confirmed by western blotting analysis of purified Fab fragment, of human IgG, under reducing conditions.

  • アプリケーション

    適用あり: Flow Cyt, ELISA, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Full length native protein (purified) (Human).

  • ポジティブ･コントロール

    • WB: Normal human immunoglobulins. Flow Cyt: Human peripheral blood cells.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 特記事項（精製）

    Purified by precipitation and chromatography

  • ポリ/モノ

    モノクローナル

  • クローン名

    4A11

  • ミエローマ

    unknown

  • アイソタイプ

    IgG2b

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Human IgG consists of four subclasses (1-4) that can be recognised by antigen differences in their heavy chains. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. The Fab fragment (fragment antigen binding) is the antigen region of the IgG molecule. It is composed of one constant and one variable domain of each of the heavy and the light chain.

画像

  • Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)
    Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)
    Flow Cytometry analysis of human peripheral blood cells labeling Human IgG Fab fragment with Anti-Human IgG Fab fragment antibody [4A11] (ab771).
  • Western blot - Anti-Human IgG Fab fragment antibody [4A11] (ab771)
    Western blot - Anti-Human IgG Fab fragment antibody [4A11] (ab771)
    All lanes : Anti-Human IgG Fab fragment antibody [4A11] (ab771)

    Lane 1 : Normal human immunoglobulins
    Lane 2 : Normal bovine immunoglobulins

    Performed under reducing conditions.
  • Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)
    Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)

    Surface staining of human peripheral blood cells with ab771. An APC-conjugated goat anti-mouse IgG was used as the secondary antibody.

参考文献

ab771 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab771 は 2 報の論文で使用されています。

  • Lau YF  et al. An adjuvant for the induction of potent, protective humoral responses to an H5N1 influenza virus vaccine with antigen-sparing effect in mice. J Virol 84:8639-49 (2010). ELISA . PubMed: 20538850
  • Drexler SK  et al. SIGIRR/TIR-8 is an inhibitor of Toll-like receptor signaling in primary human cells and regulates inflammation in models of rheumatoid arthritis. Arthritis Rheum 62:2249-61 (2010). Flow Cyt ; Human . PubMed: 20506350

レビューと Q&A

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1-8 of 8 Abreviews or Q&A

Question

Has product ab771 ever been tested for blocking exposed IgG fab fragment? Is this antibody specific for IgG fab over fc?

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Answer

We have not performed the blocking assay, but the specificity of this antibody for Fab part of IgG (over Fc) was confirmed by Western blotting using Fab fragments.

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Question

Whole human IgG adsorbed to a substrate and am looking to determine the amount of adsorbed protein that has the Fab region exposed (as opposed to the total amount of IgG adsorbed). I was planning on using immunofluorescence (preferably) or ELISA to do this, and was wondering if product ab771 would be suitable for this purpose.

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Answer

This product will recognize the Fab fragment of any exposed IgG. However if whole IgG is present and not masked by absorption into the substrate, these too will be recognized. I would recommend performing a co stain with an anti- IgG Fc specific antibody to determine whether the signal seen by ab771 is from Fabs or whole IgG. We offer a Chicken polyclonal to Human + Mouse + Rat IgG Fc fragment (ab15866) which you would be able to use as a co-stain with ab771. As this product is also against mouse Fc I would recommend sequential staining first with ab15866 followed by ab771.

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Question

We ordered this antibody. Can you provide the affinity constant KD of this antibody against Human Fab?

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Answer

Thank you for ordering the Fab fragment antibody . Unfortunately, the affinity or dissociation constant, Kd, is not determined for this antibody. If you have any other questions, please contact us.

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Question

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM High background PRIMARY ANTIBODY What is the recommended dilution to use this antibody to detect human IgG on a western blot? SECONDARY ANTIBODY What is the recommended sencondary antibody? DETECTION METHOD ECL Plus

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Answer

Thank you for your enquiry. For Western blotting, I would recommend using ab771 at a dilution of 1:1000 and optimizing based upon results. At the bottom of the online datasheet for ab771 there is a list of compatible secondary antibodies which can be used. One suggestion is ab6728 - Rabbit polyclonal to Mouse IgG H and L (HRP). If you are having problems with high background, you may want to try blocking with 3% BSA, increase the number of washes, and decreasing the concentration of primary antibody as well as the incubation period. Please contact us again if you have any additional questions.

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Question

ANTIBODY CODE ab771 PRE-SALES ENQUIRY Does it recognise IgG4 isoform and does it detect the light or heavy chain on the fab?

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Answer

Thank you for your enquiry. The antibody should recognize all human IgG isoforms. The H or L chain specificity has not been determined. If you need any further assistance please contact us again.

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Question

I want to buy IgG Fab fragment (ab771). But I need to know the isoelectric point? What is also the isoelectric point of the human IgG(ab436)? thank you for your answer,

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Answer

Thank you for your enquiry. The isoelectric point of this antibody has not been determined.

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Question

Customer would like to know if the epitopes are known for ab74987 and ab771.

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Answer

Regarding ab7498, this particular clone is Fc-specific but the epitope has not been more precisely determined. For ab771, the epitope is unknown. If you have any more questions, please contact us again.

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Question

Customer would like to know which would be better to detect human IgG - anti-Fab or anti-Fc?

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Answer

The originator of these two antibodies has informed me that the anti Fab fragment antibody is better (IgG receptors bind IgG via Fc so it may be occupied). The Fc fragment is not good for IgM bound to the B-cell surface, but is great for soluble IgM.

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