製品名Anti-Human IgG antibody [IG266]
Human IgG 一次抗体 製品一覧
製品の詳細Mouse monoclonal [IG266] to Human IgG
特異性ab200699 recognizes a protein of 75 kDa, identified as gamma heavy chain of Human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
アプリケーション適用あり: IHC-P, ICC/IF, Flow Cyt, SDS-PAGEmore details
Full length native protein (purified) corresponding to Human IgG. P01857, P01859, P01860, P01861.
- IHC-P: Human tonsil tissue.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
精製度Protein A/G purified
特記事項（精製）ab200699 is purified from bioreactor concentrate by Protein A/G.
Our Abpromise guarantee covers the use of ab200699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at RT.
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|Flow Cyt||Use 1-2µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|SDS-PAGE||Use at an assay dependent concentration.|
関連性IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
細胞内局在Cell Membrane and Secreted
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
ab200699 は 7 報の論文で使用されています。
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- Wang X et al. FKBP11 protects intestinal epithelial cells against inflammation-induced apoptosis via the JNK-caspase pathway in Crohn's disease. Mol Med Rep 18:4428-4438 (2018). PubMed: 30221722
- Wang L et al. Protein phosphatase 2A as a new target for downregulating osteoclastogenesis and alleviating titanium particle-induced bone resorption. Acta Biomater 73:488-499 (2018). PubMed: 29656074
- Yin D et al. Pro-Angiogenic Role of LncRNA HULC in Microvascular Endothelial Cells via Sequestrating miR-124. Cell Physiol Biochem 50:2188-2202 (2018). PubMed: 30415256
- Zhang Y et al. Protection of Mcc950 against high-glucose-induced human retinal endothelial cell dysfunction. Cell Death Dis 8:e2941 (2017). IP . PubMed: 28726778