Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast cancer cell line)
Permeabilization
No
Specification
Breast cancer cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/400
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Thermofisher Anti-Rabbit IgG Cross-Adsorbed
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Additional data
Additional Notes
In my experiments, ab109489 was used to detect a humanized HER2-targeted monoclonal antibody (IgG). Untreated cells had low-no staining (?background) and treated cells had strong cytoplasmic staining.
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投稿 Jul 05 2017