Anti-Human IgG 抗体 [EPR21250] (ab213242)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21250] to Human IgG
- Suitable for: ELISA, IHC-P
- Reacts with: Human
製品の概要
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製品名
Anti-Human IgG antibody [EPR21250]
Human IgG 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR21250] to Human IgG -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IHC-Pmore details -
種交差性
交差種: Human -
ポジティブ・コントロール
- IHC-P: Normal human tonsil tissue
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特記事項
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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ポリ/モノ
モノクローナル -
クローン名
EPR21250 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab213242の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 0.05 - 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 0.05 - 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
細胞内局在
Cell Membrane and Secreted -
参照データベース
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- Omim: 147100 Human
- Omim: 147110 Human
- Omim: 147120 Human
- Omim: 147130 Human
see all -
別名
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
see all
画像
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IHC image of Human IgG staining in a section of formalin-fixed paraffin-embedded normal human tonsil and normal human cerebral cortex, performed on a Leica BONDTM system using the standard protocol F. The tissue sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with ab213242, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system.
DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Tonsil tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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Different concentrations of immobilised human IgG (x-axis) was recognised in an indirect ELISA by ab213242 (1 µg x mL-1). Bound ab213242 was detected by peroxidase conjugated goat anti-rabbit IgG (1.6 µg x mL-1). OD (background signal subtracted) shown on the y-axis.
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IHC image of Human IgG staining in a section of formalin-fixed paraffin-embedded normal human tonsil, performed on a Leica BONDTM system using the standard protocol F. The tissue section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab213242, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system.
DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Tonsil tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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IHC image of Human IgG staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex, performed on a Leica BONDTM system using the standard protocol F. The tissue section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab213242, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system.
DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab213242 は論文での使用が確認できていません。