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  1. Link

    human-igg-antibody-ab2410.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Anti-Human IgG 抗体 (ab2410)

  • Datasheet
  • SDS
Submit a review Q&A (7)References (56)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody (ab2410)

    Key features and details

    • Rabbit polyclonal to Human IgG
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: unknown

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Human IgG antibody [EPR12700] (ab181236)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Human IgG antibody
      Human IgG 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to Human IgG
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: IHC-Pmore details
    • 種交差性

      交差種: Human
    • 免疫原

      Full length native protein (purified) corresponding to Human Human IgG. IgG isolated from a pool of normal human sera.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      pH: 7.3
      Preservative: 0.05% Sodium azide
      Constituent: 1% BSA
    • Concentration information loading...
    • 精製度

      Proprietary Purification
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      unknown
    • 軽鎖の種類

      unknown
    • 研究分野

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG

    関連製品

    • ChIP Related Products

      • ChIP Kit (ab500)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab2410の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    1/200 - 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
    特記事項
    IHC-P
    1/200 - 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

    ターゲット情報

    • 関連性

      IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
    • 細胞内局在

      Cell Membrane and Secreted
    • 参照データベース

      • Entrez Gene: 3500 Human
      • Entrez Gene: 3501 Human
      • Entrez Gene: 3502 Human
      • Entrez Gene: 3503 Human
      • Omim: 147100 Human
      • Omim: 147110 Human
      • Omim: 147120 Human
      • Omim: 147130 Human
      • SwissProt: P01857 Human
      • SwissProt: P01859 Human
      • SwissProt: P01860 Human
      • SwissProt: P01861 Human
      see all
    • 別名

      • FLJ39988 antibody
      • FLJ40036 antibody
      • FLJ40253 antibody
      • FLJ40587 antibody
      • FLJ40789 antibody
      • FLJ40834 antibody
      • G1m marker antibody
      • G2m marker antibody
      • G3m marker antibody
      • G4m marker antibody
      • HDC antibody
      • Heavy chain disease protein antibody
      • Human immunglobulin G antibody
      • Ig gamma 1 chain C region antibody
      • Ig gamma 2 chain C region antibody
      • Ig gamma 3 chain C region antibody
      • Ig gamma 4 chain C region antibody
      • IgG heavy chain locus antibody
      • IGHG 1 antibody
      • IGHG 2 antibody
      • IGHG 3 antibody
      • IGHG 4 antibody
      • IGHG1 antibody
      • IGHG2 antibody
      • IGHG3 antibody
      • IGHG4 antibody
      • Immunoglobulin Gm1 antibody
      • Immunoglobulin Gm3 antibody
      • Immunoglobulin Gm4 antibody
      • immunoglobulin heavy constant gamma 1 (G1m marker) antibody
      • Immunoglobulin heavy constant gamma 1 antibody
      • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
      • Immunoglobulin heavy constant gamma 2 antibody
      • immunoglobulin heavy constant gamma 3 (G3m marker) antibody
      • Immunoglobulin heavy constant gamma 3 antibody
      • immunoglobulin heavy constant gamma 4 (G4m marker) antibody
      • Immunoglobulin heavy constant gamma 4 antibody
      • MGC117419 antibody
      • MGC45809 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody (ab2410)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody (ab2410)
      ab2410 staining IgG in human tonsil sections

    プロトコール

    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (56)

    ab2410 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2410 は 56 報の論文で使用されています。

    • Li X  et al. Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes. Virol J 18:26 (2021). PubMed: 33485391
    • Li L & Zhao W The mutual regulatory loop between TPTEP1 and miR-1303 in leukemogenesis of acute myeloid leukemia. Cancer Cell Int 21:260 (2021). PubMed: 33985519
    • Bai X  et al. Activation of the eIF2a/ATF4 axis drives triple-negative breast cancer radioresistance by promoting glutathione biosynthesis. Redox Biol 43:101993 (2021). PubMed: 33946018
    • Lv M & Liu Q JMJD2C triggers the growth of multiple myeloma cells via activation of ß-catenin. Oncol Rep 45:1162-1170 (2021). PubMed: 33469678
    • Miao Y  et al. Histone methyltransferase SUV39H2 regulates LSD1-dependent CDH1 expression and promotes epithelial mesenchymal transition of osteosarcoma. Cancer Cell Int 21:2 (2021). PubMed: 33397384
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Question

    Has this antibody ever been tested for IHC-P to detect immune deposits in human skin biopsies for the diagnosis of autoimmune diseases like lupus erythematosus?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 11 2012

    Answer

    We have not received any report of the this antibody being used for diagnosis of autoimmune diseases by IHC or other methods. It has been demonstrated to be capable of staining a sub-population of cells in tonsil sections, as shown on the datasheet, but detection of immune deposits of immunoglobulins may requirea more sensitive tecnique. If you do decide to try this antibody, please contact us if you have any difficulty.

    Read More

    Abcam Scientific Support

    Answered on Jul 11 2012

    Question

    Ab2410 does not bind to human IgG light chain on a Western blot, but it does bind to the heavy chain.
    Looking for a rabbit anti-human IgG (H&L) antibody, a mouse anti-human IgG (H&L) antibody, and an anti-mouse IgG (H&L) antibody conjugated to AP.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 10 2012

    Answer

    Thank you for your call today and for your questions.

    Here are the products that we discussed:

    1) Rabbit anti-human IgG (H&L) antibody: Ab6715

    https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Human-IgG-H-L-ab6715.html

    2) Mouse anti-human IgG (H&L) antibody: Ab34834

    https://www.abcam.com/Mouse-polyclonal-Secondary-Antibody-to-Human-IgG-H-L-ab34834.html

    3) Anti-mouse IgG (H&L) antibody conjugated to AP: Ab7069

    https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7069.html

    This anti-mouse IgG antibody is pre-adsorbed against human IgG, so that it will not bind to the human IgG on the Western blot. It will only bind to the mouse primary antibody.

    I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you. Have a nice day.

    Read More

    Abcam Scientific Support

    Answered on Jul 10 2012

    Question

    Yes, if you can please generate me a copy of an MSDS that would be helpful.



    Thanks and regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 27 2012

    Answer

    Thank you for your reply.

    I attached the MSDS to this email for your information.

    I hope this ids helpful and wish you good luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Jun 27 2012

    Question

    Hello,



    We received product ab2410 lot #GR74515 Rabbit polyclonal to Human IgG-ChIP Grade. We need an MSDS for our record for any reagents that we receive. Please e-mail me a copy of the MSDS.



    Thanks and regards,







    This email and any attachments contain confidential information. This is a private communication. If you have received this email in error, please notify the sender immediately and do not disclose the contents to another person, use it for another purpose, or store or copy the information in any medium.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 26 2012

    Answer

    Thank you for your inquiry.

    The MSDS PDFsfor our products can be found on the online datasheets.

    If there is not MSDS as in the case of ab2410, this indicates that the product does not contains hazardous substances or only contains so little that a MSDS is not necessary.

    If you still require a MSDS for ab2410, I will be happy to ask the department to generate one for you (which would say, that it is not hazardous in these minute amounts).

    I hope this information is helpful and wish you a good day.

    Read More

    Abcam Scientific Support

    Answered on Jun 26 2012

    Question

    für folgende Antikörper möchte ich eine entsprechende Isotypkontrolle bestellen, um diese bei der Färbung mitzuführen:
    Anti-IL9 antibody (https://www.abcam.com/IL-9-antibody-ab111915.html)
    Anti-IL9R antibody (https://www.abcam.com/IL-9R-antibody-ab106287.html)
    Folgende Isotypkontrollen habe ich auf Ihrer Homepage gefunden:
    Anti-Human IgG antibody (ab2410)
    Anti-Human IgG antibody, prediluted (ab938)
    Sind dies Isotypkontrollen, die für den IL-9 und IL-9R Antikörper empfohlen werden und bei der Etablierung der Färbung mitgeführt wurden?
    Vielen Dank im Voraus.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 25 2012

    Answer

    Vielen Dank für Ihre Nachrichten.

    Leider sind die anti-human IgGs nicht die Kontrollen, die wir Ihnen als Isotypkontrolle für die IL9- und IL9R- Antikörper empfehlen würden. (Damit würden Sie sicher eine Färbung erhalten, die Sie so nicht beabsichtigen).

    Als Isotypkontrolle können wir Ihnen ab27478 oder ab27472 (vorverdünnt=prediluted) empfehlen:

    https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27478.html (or use the following: https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27478.html).

    https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27472.html (or use the following: https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27472.html).

    Es freut mich zu hören, dass Sie unser Angebot unsere Antikörper ab106287und ab111915an Gefrierschnitten zu testen, annehmen möchten.



    Wie besprochen, können Sie den Gutscheincode zum Kauf jeweils eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in IHC-Fr eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

    Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

    Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: https://www.abcam.com/collaborationdiscount

    Read More

    Abcam Scientific Support

    Answered on Apr 25 2012

    Question

    IHC-Fr, injecting human cells into mouse likes to detect human cells only, but not mouse was mouse cross reactivity tested / homology?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 07 2011

    Answer

    Thank you for contacting us. I have heard back from the lab with the following information: This antibody was only tested on human tissues and not on any other species. Therefore, we do not know if it would crossreact with mouse or not. I am sorry to not have more helpful information for you in this regard. Please let me know if you would be looking into other proteins that might show low homology between human and mouse, and I can help you to find a suitable antibody. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Dec 07 2011

    Question

    I'm using this antibody to stain monkey tissue in IHC-P at 1/2000 with pepsin antigen retrieval.  I'm seeing some background though and my negative isotype control shows signal too.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 02 2011

    Answer

    Thank you for contacting us and I'm sorry to hear that you're having some trouble staining with ab2410. We haven't tested this antibody using monkey tissue, only human tissue (tonsil), so we haven't optimized the protocol for monkey. However, I've included some info about the protocol tested for you to try optimizing. Pretreatment enzymatic retrieval: We recommend histozyme treatment (Diagnostic Biosystem cat. #: K 046) for ab2410 for 5 minutes at room temperature. The antibody should be diluted even further if you're seeing background. Try 1/3000 - 1/5000 and dilute your secondary more too. The lab suggests incubating the primary for 30 mins at room temperature. Plus, what are you using for blocking? We'd recommend 5-10% serum from the species your secondary is raised in and incubating that for 1 hr at RT. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Nov 02 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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