Human IgD ELISA Kit (ab157708)
Key features and details
- Sensitivity: 4.4545 ng/ml
- Range: 6.25 ng/ml - 400 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IgD ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall < 10% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall < 10% -
サンプルの種類
Serum, Plasma, Other biological fluids -
アッセイタイプ
Sandwich (quantitative) -
検出感度
4.4545 ng/ml -
検出範囲
6.25 ng/ml - 400 ng/ml -
添加回収試験
> 85 %
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ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IgD Human ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgD in Human samples.
In this assay the IgD present in samples reacts with the anti-IgD antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgD antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgD. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgD in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgD in the test sample. The quantity of IgD in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 100X HRP-conjugated anti-Human IgD antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Human IgD Calibrator (lyophilized) 1 vial Human IgD ELISA Microplate 1 unit Stop Solution 1 x 12ml -
研究分野
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関連性
Little is known about the normal function of IgD, and few clinical signs or symptoms are associated with its absence. Individuals with low or absent levels of IgD do not appear unusually predisposed to infections. In mice, IgD may substitute for some functions of IgM when IgM is absent. IgD is expressed on peripheral B cells and is used as a pan B cell marker. -
別名
- IGHD
- Immunoglobulin heavy constant delta
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参照データベース
- Entrez Gene: 3495 Human
- Omim: 147170 Human
- SwissProt: P01880 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab157708 は論文での使用が確認できていません。