Human I-309 ELISA Kit (CCL1) (ab100536)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 4.12 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human I-309 ELISA Kit (CCL1)
CCL1 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 4 pg/ml -
検出範囲
4.12 pg/ml - 3000 pg/ml -
添加回収試験
95 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 79.9 70% - 103% Plasma 97.65 88% - 110% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s I-309 (CCL-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human I-309 (CCL-1) in plasma and cell culture supernatants. (Human I-309 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human I-309 coated on a 96-well plate. Standards and samples are pipetted into the wells and I-309 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human I-309 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-309 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human I-309 in normal serum with ab100536, only in serum spiked with Human I-309.
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特記事項
Optimization may be required with urine samples
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-Human I-309 2 vials I-309 Microplate (12 x 8 wells) 1 unit Recombinant Human I-309 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C-C motif chemokine 1
- Ccl1
- CCL1_HUMAN
see all -
参照データベース
- Entrez Gene: 6346 Human
- Omim: 182281 Human
- SwissProt: P22362 Human
- Unigene: 72918 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab100536 は 1 報の論文で使用されています。
- Fujikawa M et al. Chemokine (C-C Motif) Ligand 1 Derived from Tumor-Associated Macrophages Contributes to Esophageal Squamous Cell Carcinoma Progression via CCR8-Mediated Akt/Proline-Rich Akt Substrate of 40 kDa/Mammalian Target of Rapamycin Pathway. Am J Pathol 191:686-703 (2021). PubMed: 33460563