Human Histone H2A.Z peptide (ab11681)
Key features and details
- Purity: > 90% HPLC
- Suitable for: Blocking
製品の詳細
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製品名
Human Histone H2A.Z peptide
Histone H2A.Z タンパク質・ペプチド 製品一覧 -
精製度
> 90 % HPLC. -
アクセッション番号
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Animal free
No -
由来
Synthetic -
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生物種
Human
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関連製品
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Corresponding Antibody
特性
Our Abpromise guarantee covers the use of ab11681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Blocking - Blocking peptide for Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)
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製品の状態
Liquid -
備考
Synthetic peptide: C-SLIGKKGQQKT, corresponding to amino acids 116-126 of Human H2A.Z.- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
関連情報
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別名
- H2A histone family member Z
- H2A.z
- H2A/z
see all -
機能
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. -
配列類似性
Belongs to the histone H2A family. -
翻訳後修飾
Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Not phosphorylated. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
画像
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Western blot - Human Histone H2A.Z peptide (ab11681)All lanes : Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) at 1/500 dilution
Lanes 1 & 3 : Calf thymus histone lysate
Lanes 2 & 4 : Calf thymus histone lysate withHuman Histone H2A.Z peptide (ab11681) at 1 µg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Observed band size: 15 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab11681 は 1 報の論文で使用されています。
- Kafer GR et al. In Vitro Manipulation of Mammalian Preimplantation Embryos Can Alter Transcript Abundance of Histone Variants and Associated Factors. Cell Reprogram : (2011). WB . PubMed: 21827322
