Human Hemopexin ELISA Kit (HPX) (ab108859)
Key features and details
- Sensitivity: 70 ng/ml
- Range: 0.078 µg/ml - 20 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
-
製品名
Human Hemopexin ELISA Kit (HPX)
Hemopexin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.8% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Competitive -
検出感度
> 70 ng/ml -
検出範囲
0.078 µg/ml - 20 µg/ml -
添加回収試験
102 %
-
全工程の試験時間
2h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Hemopexin (HPX) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of hemopexin levels in plasma and serum.
A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Hemopexin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Hemopexin captured in plate.
Get results in 90 minutes with Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 2X Biotinylated Human Hemopexin (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml Hemopexin Microplate (12 x 8 well strips) 1 unit Hemopexin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
-
機能
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. -
組織特異性
Expressed by the liver and secreted in plasma. -
配列類似性
Belongs to the hemopexin family.
Contains 5 hemopexin-like domains. -
翻訳後修飾
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- Beta 1B glycoprotein
- Beta-1B-glycoprotein
- FLJ56652
see all -
参照データベース
- Entrez Gene: 3263 Human
- Omim: 142290 Human
- SwissProt: P02790 Human
- Unigene: 426485 Human
画像
-
Competitive ELISA - Hemopexin (ab108859
Hemopexin measured in various biological fluids showing quantity (μg) per mL of tested sample
-
Typical Standard CurveRepresentative Standard Curve using ab108859.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (5)
ab108859 は 5 報の論文で使用されています。
- Paul S et al. Plasma protein(s)-based conceptual diagnostic tool for assessing high-altitude acclimation in humans. Funct Integr Genomics 20:191-200 (2020). PubMed: 31444657
- Canesin G et al. Scavenging of Labile Heme by Hemopexin Is a Key Checkpoint in Cancer Growth and Metastases. Cell Rep 32:108181 (2020). PubMed: 32966797
- Lawson HA et al. Physiologic and genetic evidence links hemopexin to triglycerides in mice and humans. Int J Obes (Lond) 41:631-638 (2017). PubMed: 28119529
- Evans C et al. Impairment of neutrophil oxidative burst in children with sickle cell disease is associated with heme oxygenase-1. Haematologica N/A:N/A (2015). PubMed: 26315932
- Insenser M et al. Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism: a nontargeted proteomic study. PLoS One 9:e100902 (2014). PubMed: 24979072