Human GSDMD ELISA Kit (ab272463)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.154 ng/ml
- Range: 0.78 ng/ml - 50 ng/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human GSDMD ELISA Kit
GSDMD キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Extract 8 2.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Extract 3 8.8% -
サンプルの種類
Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.154 ng/ml -
検出範囲
0.78 ng/ml - 50 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell Lysate 103 95% - 117% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human GSDMD ELISA kit (ab272463) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human GSDMD protein in cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate GSDMD with 0.154 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
GSDMD is a part of a family of highly conserved proteins expressed primarily in epithelial tissues. GSDMD is activated by caspases in response to inflammasome activators where it drives pyroptosis during microbial infection and danger signals. Down regulation of GSDMD is found in gastric cancer and mutations in GSDMD have been found in a variety of cancers.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human GSDMD Capture Antibody 1 x 600µl 10X Human GSDMD Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human GSDMD Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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配列類似性
Belongs to the gasdermin family. - Information by UniProt
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別名
- 1810036L03Rik
- DF 5L
- DF5L
see all -
参照データベース
- Entrez Gene: 79792 Human
- Omim: 617042 Human
- SwissProt: P57764 Human
- Unigene: 118983 Human
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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The GSDMD standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of GSDMD were measured in duplicate and interpolated from the GSDMD standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GSDMD concentration was determined to be 25.76 ng/mL in A549, 30.24 ng/ml in Jurkat, and 9.19 ng/ml in liver homogenate extract.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab272463 は 5 報の論文で使用されています。
- Planès R et al. Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells. Mol Cell 82:2385-2400.e9 (2022). PubMed: 35594856
- Silva CMS et al. Gasdermin-D activation by SARS-CoV-2 triggers NET and mediate COVID-19 immunopathology. Crit Care 26:206 (2022). PubMed: 35799268
- Arik E et al. Erythropoietin Enhances Post-ischemic Migration and Phagocytosis and Alleviates the Activation of Inflammasomes in Human Microglial Cells. Front Cell Neurosci 16:915348 (2022). PubMed: 35813499
- Ye X et al. GSDMD contributes to myocardial reperfusion injury by regulating pyroptosis. Front Immunol 13:893914 (2022). PubMed: 36217543
- Zhao J et al. Disulfiram alleviates acute lung injury and related intestinal mucosal barrier impairment by targeting GSDMD-dependent pyroptosis. J Inflamm (Lond) 19:17 (2022). PubMed: 36266722