Human GDF-15 ELISA Kit (ab155432)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 1.1 pg/ml - 800 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human GDF-15 ELISA Kit
GDF15 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall < 10% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall < 12% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2 pg/ml -
検出範囲
1.1 pg/ml - 800 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 104.2 92% - 116% Plasma 102.9 88% - 119% Cell culture media 114.2 96% - 105% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human
交差が予測される動物種: Non human primates -
製品の概要
Abcam's GDF-15 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human GDF-15 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human GDF-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and GDF-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GDF-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GDF-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-Human GDF-15 2 vials GDF-15 Microplate (12 x 8 wells) 1 unit Recombinant Human GDF-15 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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組織特異性
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- GDF 15
- GDF-15
- Gdf15
see all -
参照データベース
- Entrez Gene: 9518 Human
- Omim: 605312 Human
- SwissProt: Q99988 Human
- Unigene: 616962 Human
画像
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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GDF-15 measured in cell culture supernatants showing quantity (ng) per mL of tested sample. Samples were diluted 20-540 fold.
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GDF-15 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 2-54 fold.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab155432 は 6 報の論文で使用されています。
- Li P et al. Growth Differentiation Factor 15 Protects SH-SY5Y Cells From Rotenone-Induced Toxicity by Suppressing Mitochondrial Apoptosis. Front Aging Neurosci 14:869558 (2022). PubMed: 35721026
- Zhou B et al. GDF15 serves as a coactivator to enhance KISS-1 gene transcription through interacting with Sp1. Carcinogenesis 42:294-302 (2021). PubMed: 32966555
- Wei W et al. Difference in inflammation, atherosclerosis, and platelet activation between coronary artery aneurysm and coronary artery ectasia. J Thorac Dis 12:5811-5821 (2020). PubMed: 33209413
- Belo L et al. Hepcidin and diabetes are independently related with soluble transferrin receptor levels in chronic dialysis patients. Ren Fail 41:662-672 (2019). PubMed: 31296086
- Zhang Y et al. Sex-specific differences in the modulation of Growth Differentiation Factor 15 (GDF15) by hyperoxia in vivo and in vitro: Role of Hif-1a. Toxicol Appl Pharmacol 332:8-14 (2017). PubMed: 28734801
- Zhou YM et al. Growth differentiation factor-15 (GDF-15), novel biomarker for assessing atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease. Int J Clin Exp Med 8:21201-7 (2015). PubMed: 26885055