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Human GBP1 ELISA Kit (ab270897)

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SimpleStep ELISA Protocol Diagram
  • Example of human GBP1 standard curve in Sample Diluent NS.
  • Interpolated concentrations of native GBP1 in human plasma samples.
  • Sandwich ELISA - Human GBP1 ELISA Kit (ab270897)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 24.09 pg/ml
  • Range: 109.38 pg/ml - 7000 pg/ml
  • Sample type: Cit plasma, Hep Plasma
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human GBP1 ELISA Kit
    GBP1 キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Plasma 8 8.3%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Plasma 3 8.7%

  • サンプルの種類

    Hep Plasma, Cit plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    24.09 pg/ml

  • 検出範囲

    109.38 pg/ml - 7000 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Hep Plasma 85 82% - 87%
    Cit plasma 99 91% - 104%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Human GBP1 ELISA kit (ab270897) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GBP1 protein in human citrate and heparin plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate human GBP1 with 24.09 ng/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 特記事項

    Human Guanylate-binding protein 1 (GBP1) is a 67 kDa GTPase. Protein expression is induced by response to interferon gamma. GBP1 hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. It has been reported to have antiviral activity against the influenza virus. In addition, GBP1 has been shown to promote oxidative killing and delivery of antimicrobial peptides the autophagolysosomes, providing broad host protection against different pathogen classes. Human GBP1 shares 69%, 72%, 22% sequence identity with mouse, rat, and cow, respectively.

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Human GBP1 Capture Antibody 1 x 600µl
    10X Human GBP1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human GBP1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • 研究分野

  • 機能

    Binds GTP, GDP and GMP.

  • 配列類似性

    Belongs to the GBP family.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 別名

    • GBP 1
    • GBP-1
    • GBP1
    • GBP1_HUMAN
    • GTP binding protein 1
    • GTP-binding protein 1
    • Guanine nucleotide binding protein 1
    • Guanine nucleotide-binding protein 1
    • Guanylate binding protein 1
    • Guanylate binding protein 1 interferon inducible
    • Guanylate binding protein 1 interferon inducible 67kDa
    • HuGBP 1
    • HuGBP-1
    • HuGBP1
    • Interferon induced guanylate binding protein 1
    • Interferon-induced guanylate-binding protein 1
    • OTTHUMP00000012352
    see all

  • 参照データベース

    画像

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Example of human GBP1 standard curve in Sample Diluent NS.
      Example of human GBP1 standard curve in Sample Diluent NS.

      The GBP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native GBP1 in human plasma samples.
      Interpolated concentrations of native GBP1 in human plasma samples.

      The concentrations of GBP1 were measured in duplicates, interpolated from the GBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 50% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GBP1 concentration was determined to be 1182.48 pg/mL in plasma (citrate) and 789.88 pg/mL in and plasma (heparin).

    • Sandwich ELISA - Human GBP1 ELISA Kit (ab270897)
      Sandwich ELISA - Human GBP1 ELISA Kit (ab270897)
      To learn more about the advantages of recombinant antibodies see here.

    参考文献 (0)

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    ab270897 は論文での使用が確認できていません。

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