Human FX ELISA kit (total FX antigen) (ab272773)
Key features and details
- Range: 0.1 ng/ml - 50 ng/ml
- Sample type: Cit plasma, EDTA Plasma
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
製品の概要
-
製品名
Human FX ELISA kit (total FX antigen)
Factor X キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Sample 1 20 0.71ng/ml 0.039 5.44% Sample 2 20 2.97ng/ml 0.155 5.23% Sample 3 20 12.63ng/ml 0.095 2.45% -
サンプルの種類
EDTA Plasma, Cit plasma -
アッセイタイプ
Quantitative -
検出範囲
0.1 ng/ml - 50 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Spike 98 % - % -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human FX ELISA kit (total FX antigen) (ab272773) is intended for the quantitative determination of total Factor X antigen in human plasma.
Human Factor X will bind to the capture antibody coated on the microtiter plate. Factor X and Xa will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human Factor X primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor X present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor X. Color development is proportional to the concentration of Factor X in the samples.
Factor X standard provided is calibrated against the WHO 4th International Standard.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Human FX Primary Antibody Lyophilized Vial 1 vial Anti-Rabbit HRP Secondary Reagent 1 vial FX ELISA Plate 1 unit Human FX Standard Lyophilized Vial 1 vial TMB Substrate 1 x 10ml -
研究分野
-
機能
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. -
組織特異性
Plasma; synthesized in the liver. -
関連疾患
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. -
配列類似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
翻訳後修飾
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- Activated factor Xa heavy chain
- Coagulation factor X
- F10
see all -
参照データベース
- Entrez Gene: 2159 Human
- Omim: 227600 Human
- SwissProt: P00742 Human
- Unigene: 361463 Human
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab272773 は論文での使用が確認できていません。