製品の概要

  • 製品名

    Human Fibrinogen ELISA Kit
    Fibrinogen キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8.6%
  • サンプルの種類

    Cell culture supernatant, Saliva, Milk, Urine
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    = 1 ng/ml
  • 検出範囲

    1.25 ng/ml - 80 ng/ml
  • 添加回収試験

    96 %

  • 全工程の試験時間

    4h 00m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Human
  • 製品の概要

    Human Fibrinogen ELISA kit is designed for the quantitative measurement of fibrinogen concentrations in cell culture media, saliva, milk and urine samples.


    A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.


    Get better reproducibility in only 90 minutes with Human Fibrinogen ELISA Kit (ab208036) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Fibrinogen Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Fibrinogen Microplate (12 x 8 well strips) 1 unit
    Fibrinogen Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究分野

  • 機能

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • 組織特異性

    Plasma.
  • 関連疾患

    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • 配列類似性

    Contains 1 fibrinogen C-terminal domain.
  • ドメイン

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • 翻訳後修飾

    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • FGA
    • FGB
    • FGG
    • Fib2
    • FIBA_HUMAN
    • Fibrinogen A alpha polypeptide
    • Fibrinogen alpha chain
    • Fibrinogen B alpha polypeptide
    • Fibrinogen beta chain
    • Fibrinogen G alpha polypeptide
    • Fibrinogen gamma chain
    • fibrinogen, B beta polypeptide
    • fibrinogen, G gamma polypeptide
    • fibrinogen, gamma polypeptide
    • Fibrinogen--alpha -polypeptide chain
    • Fibrinogen--beta -polypeptide chain
    • Fibrinogen--gamma-polypeptide chain
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

画像

  • Representative Standard Curve using ab108841.

プロトコール

参考文献

This product has been referenced in:

  • Zhang Y  et al. Application of Isobaric Tags for Relative and Absolute Quantification (iTRAQ) Coupled with Two-Dimensional Liquid Chromatography/Tandem Mass Spectrometry in Quantitative Proteomic Analysis for Discovery of Serum Biomarkers for Idiopathic Pulmonary Fibrosis. Med Sci Monit 24:4146-4153 (2018). Read more (PubMed: 29909421) »
  • Wang H  et al. Urinary Fibrinogen as a Predictor of Progression of CKD. Clin J Am Soc Nephrol 12:1922-1929 (2017). Read more (PubMed: 28903970) »
See all 3 Publications for this product

レビューと Q&A

1-5 of 5 Abreviews or Q&A

Cross reactive with Rhesus EDTA plasma samples

Excellent Excellent 5/5 (Ease of Use)
Abreviews
An experience laboratory technician completed this Fibrinogen ELISA from Abcam, catalog number AB108841, lot GR245646-10. The goal of the experiment was to determine the cross-reactivity of this kit for EDTA-prepared plasma samples and serum samples for M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius.

Overall, the kit, like other Abcam ELISA kits, was easy to follow and straight-forward. The laboratory technician had no problem understanding any parts of the protocol.

Our test was performed in 2 basic steps. The first was to find the appropriate testable dilution in plasma and serum, and the second was to test the linearity and spike recovery.

To this end our first experiment tested 8 dilutions of both serum and plasma ranging from 1:10 to 1:640 (8 1:2 serial dilutions). In these dilution ranges, the serum was well within the OD range, however no linearity was observed in any part of the dilution scheme. The EDTA plasma was beyond the range of detection (under-diluted).

Our second experiment focused on the EDTA prepared plasma. We diluted n=2 rhesus plasma samples 1:10,000, 1:30,000, 1:90,000, and 1:270,000 and tested in duplicate (as were the standards). The rest of the assay was performed to protocol’s specifications. For both animals tested, 1:90,000 and 1:270,000 gave similar results, suggesting this is the linear range of the assay.

Absorbance measurements of the diluted samples were comparable between duplicates in both tests (pipetting error appeared to be at a minimum). We also performed a spike recovery in both pure diluent and diluted plasma. ~100% of spiked human Fibrinogen was detected in the 90,000 and 270,000 diluted samples.

Overall, we recommend this kit for detection of M. mulatta fibrinogen in EDTA-prepared plasma.

Abcam user community

Verified customer

投稿 Jun 07 2016

Answer

Plasma treated with EDTA anticoagulant can be used with all three of these ELISA kits. However, if choosing between ab108842 and ab108841, the competitive ELISA ab108842 is preferable to ab108841 for this sample type. The SimpleStep ELISA ab171578 is preferable to both for plasma samples, given the relative simplicity of the protocol and the data for plasma samples that is presented on the datasheet.

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Question
Answer



For this lot, there are 400 ng of standard per vial. So, reconstitute in 5 mL of the diluent for a 80 ng/mL standard solution.

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Answer

Thank you for you for your enquiry and your interest in our products.

I have conducted a search for you using our advanced search engine. Currently, we only have ELISA kits which are specific for either human (ab108841, ab108842) or mouse fibrinogen (ab108843, ab10844). Unfortunately, we do not have any kits which would recognize both species.

Apologies!

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for your inquiry. ab108884 is an alpha 1 microglobulin human ELISA kit, Swiss Prot P02760. Alpha1-microglobulin (1M), also called protein HC, is a tubular plasma and tissue protein that belongs to the lipocalin transport protein superfamily for small hydrophobic molecules. It contains 184 amino acids and weighs 26-kDa (1-2). Mature 1M and bikunin (urinary trypsin inhibitor) result from a common precursor (3). (1) Grubb AO et al. (1983) Biol Chem. 258:14698-14707 (2) Ekström B and Berggård I. (1977) J Biol Chem 252:8048-8057 (3) Vetr H and Gebhard W (1990) Biol Chem Hoppe Seyler. 371:1185-1196 https://www.abcam.com/alpha-1-Microglobulin-Human-ELISA-Kit-ab108884.html http://www.uniprot.org/uniprot/P02760#PRO_0000017886 ab108883 is an is an alpha 2 macroglobulin human ELISA kit, Swiss Prot P01023. It's a major serum protein. (1) Pineda-Salgado L et al (2005) Gene Expr Patterns. 6(1):3-10 (2) Jensen PE et al (2004) Biocim Biophys Acta. 5; 1690(3): 203-7 (3) Yang AH et al (1997) Nephrol Dial Transplant 12(3): 465-9 (4) Shiota G et al (1995) J Med 26(5-6): 295-308. https://www.abcam.com/alpha-2-Macroglobulin-Human-ELISA-Kit-ab108883.html http://www.uniprot.org/uniprot/P01023 ab108823 is a complement C3 human ELISA kit, Swiss Prot P01024.  Complement protein C3 is the fourth component to attach in the complement reaction sequence. It is a beta-globulin with a sedimentation coefficient of 5.5 and a molecular weight of 185,000. (1) Sahu A et al. (2001) Immunol. Rev. 180: 35-48 (2) Sacks S et al. 92003) J Mol Med. 81(7): 404-10 (3) Ramos-Casals M et al. (2004) Rheumatology (Oxford). (4) Gardinali M et al. (1998) Clin.Immunol.Immunopathol .87(3): 97-303 https://www.abcam.com/Complement-C3-Human-ELISA-kit-ab108823.html http://www.uniprot.org/uniprot/P01024 ab108841 is a fibrinogen human ELISA kit, Swiss Prot P02671, P02675, and P02679.  Fibrinogen (FBG) is a homodimer of molecular mass 340 kDa, made up of two sets of α, β, γ polypeptide chains, and synthesized in the parenchymal cell of the hepatocyte and in the megakaryocyte (1). FBG plays a major role in coagulation, and both elevated and decreased levels have clinical significance. Upon cleavage by thrombin in the initial stages of coagulation activation, FBG self-assembles to yield a fibrin clot matrix that subsequently is crosslinked by factor XIIIa to form an insoluble network. FBG also binds to the platelet glycoprotein IIbIIIa receptor so as to form bridges between platelets, thus facilitating aggregation (2). Elevated plasma FBG has been identified as an independent risk factor for coronary atherosclerosis and ischemic heart disease (3, 4). Individuals with congenital absence of FBG, termed afibrinogenemia, have prolonged bleeding times. (1) Doolittle, R.F. (1984) Annu. Rev. Biochem 53:195 (2) Handley, D.A. and Hughes, T.E. (1997) Thromb. Res. 87:1 (3) Handa, K. et al. (1989) Atherosclerosis 77:209 (4) Mannucci, P.M. and Mari, D. (1993) Fibrinolysis 3:51 (5) Amiral J. (1995) Clin. Appl. Thrombosis Hemostasis 1:243 http://www.uniprot.org/uniprot/P02671 http://www.uniprot.org/uniprot/P02675 http://www.uniprot.org/uniprot/P02679 I hope this information helps.  Please contact us with any other questions.

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