Human Ferritin (FTL) ELISA Kit (ab108837)
Key features and details
- Sensitivity: 0.56 ng/ml
- Range: 0.781 ng/ml - 50 ng/ml
- Sample type: Cell Lysate, Cerebral Spinal Fluid, Milk, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Ferritin (FTL) ELISA Kit
Ferritin Light Chain キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.7% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.4% -
サンプルの種類
Milk, Urine, Serum, Plasma, Cell Lysate, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.56 ng/ml -
検出範囲
0.781 ng/ml - 50 ng/ml -
添加回収試験
95 %
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全工程の試験時間
4h 0m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human
交差が予測される動物種: Monkey
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製品の概要
Abcam’s Ferritin (FTL) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ferritin concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture supernatants.
A Ferritin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ferritin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ferritin captured in plate.
Get results in 90 minutes with Human Ferritin ELISA Kit (FLT) (ab200018) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Ferritin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Ferritin Microplate (12 x 8 well strips) 1 unit Ferritin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
関連疾患
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. -
配列類似性
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
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別名
- Ferritin L chain
- Ferritin L subunit
- Ferritin light chain
see all -
参照データベース
- Entrez Gene: 2512 Human
- Omim: 134790 Human
- SwissProt: P02792 Human
- Unigene: 433670 Human
- Unigene: 713706 Human
画像
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Sandwich ELISA - Ferritin (FTL) Human ELISA Kit (ab108837)
Human Ferritin measured in biological fluids showing quantity (pg) per mL of tested sample. Serum and plasma samples were diluted 10-20 fold. Milk, urine and PBMC supernatant were diluted 1-4 fold.
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Sandwich ELISA - Ferritin (FTL) Human ELISA Kit (ab108837)Human Ferritin standard curve: mean of duplicates (+/- SD) -
Typical Standard CurveRepresentative Standard Curve using ab108837
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab108837 は 15 報の論文で使用されています。
- Martínez-Soto JM et al. Increased serum ferritin is associated with oxidized low-density lipoprotein in prediabetes patients: A pilot study. Heliyon 7:e06720 (2021). PubMed: 33912708
- Hu Y et al. Clinical Characteristics, Iron Metabolism and Neuroinflammation: New Insight into Excessive Daytime Sleepiness in Parkinson's Disease. Neuropsychiatr Dis Treat 17:2041-2051 (2021). PubMed: 34188474
- Lian TH et al. Tremor-Dominant in Parkinson Disease: The Relevance to Iron Metabolism and Inflammation. Front Neurosci 13:255 (2019). PubMed: 30971879
- Choi EK et al. Ferroportin disease mutations influence manganese accumulation and cytotoxicity. FASEB J 33:2228-2240 (2019). PubMed: 30247984
- Allden SJ et al. The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med N/A:N/A (2019). PubMed: 31051082