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Human Ferritin ELISA Kit (ab108698)

Reviews (1)Q&A (2)References (4)
Typical Standard Curve

    Key features and details

    • Sensitivity: 0.53 ng/ml
    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    製品の概要

    • 製品名

      Human Ferritin ELISA Kit
      Ferritin Light Chain キット 製品一覧

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Control 48 < 5.4%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Control 48 < 6.1%

    • サンプルの種類

      Serum, Hep Plasma, Cit plasma

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      0.53 ng/ml

    • 添加回収試験

      98.66 %

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Human

    • 製品の概要

      Human Ferritin ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Ferritin (FTL) in serum and plasma.


      A 96-well plate has been precoated with anti-Ferritin IgG antibodies. Samples, standards and the Ferritin-HRP conjugate are added to the wells, where Ferritin in the sample and standards binds to the precoated antibody and added Ferritin-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Ferritin in the sample and the intensity is measured at 450 nm.


      Get results in 90 minutes with Human Ferritin ELISA Kit (FLT) (ab200018) from our SimpleStep ELISA® range.

    • 試験プラットフォーム

      Microplate

    製品の特性

    • 保存方法

      Store at +4°C. Please refer to protocols.
    • 内容 ラベル 1 x 96 tests
      10X Washing Solution 1 x 50ml
      Anti-Ferritin IgG Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit
      Anti-Ferritin-HRP conjugate 1 x 12ml
      Cover foil 1 unit
      Ferritin Control 1 x 1ml
      Ferritin Standard 0 - 0 ng/mL 1 x 3ml
      Ferritin Standard 1 - 5 ng/mL 1 x 1ml
      Ferritin Standard 2 - 20 ng/mL 1 x 1ml
      Ferritin Standard 3 - 100 ng/mL 1 x 1ml
      Ferritin Standard 4 - 400 ng/mL 1 x 1ml
      Ferritin Standard 5 - 1,000 ng/mL 1 x 1ml
      Stop Solution 1 x 15ml
      Strip holder 1 unit
      TMB Substrate Solution 1 x 15ml

    • 研究分野

    • 機能

      Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.

    • 関連疾患

      Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
      Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.

    • 配列類似性

      Belongs to the ferritin family.
      Contains 1 ferritin-like diiron domain.
    • Information by UniProt

    • 別名

      • Ferritin L chain
      • Ferritin L subunit
      • Ferritin light chain
      • Ferritin light polypeptide
      • ferritin light polypeptide like 3
      • FRIL_HUMAN
      • FTL
      • LFTD
      • NBIA 3
      • NBIA3
      see all

    • 参照データベース

      関連製品

      画像

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab108698

      参考文献 (4)

      ab108698 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab108698 は 4 報の論文で使用されています。

      • Raha AA  et al. Hepcidin Increases Cytokines in Alzheimer's Disease and Down's Syndrome Dementia: Implication of Impaired Iron Homeostasis in Neuroinflammation. Front Aging Neurosci 13:653591 (2021). PubMed: 33994996
      • Ajepe AA  et al. Prevalence and foetomaternal effects of iron deficiency anaemia among pregnant women in Lagos, Nigeria. PLoS One 15:e0227965 (2020). PubMed: 31971986
      • Devos D  et al. A ferroptosis-based panel of prognostic biomarkers for Amyotrophic Lateral Sclerosis. Sci Rep 9:2918 (2019). PubMed: 30814647
      • Smith EM  et al. High-dose vitamin D3 reduces circulating hepcidin concentrations: A pilot, randomized, double-blind, placebo-controlled trial in healthy adults. Clin Nutr 36:980-985 (2017). PubMed: 27402475

      レビューと Q&A

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      1-3 of 3 Abreviews or Q&A

      Human Ferritin ELISA Kit for Human Cerebrospinal Fluid

       Excellent Excellent 5/5 (Ease of Use)
      Abreviews
      abreview image
      We used the Human Ferritin ELISA kit (Cat# ab108698) to measure the ferritin levels in the cerebrospinal fluids (CSF) of human patients when and after subarachnoid hemorrhage happens.
      It works well with the data that the ferritin in human CSF on the day of SAH is ~10 ng/mL, and dramatically increased higher than 100 even close to 4,000 ng/mL, which are matched with the results in published literature.
      We used the standards ranged from 5 to 1,000 ng/mL, and diluted the CSF samples to fit the range.
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jan 20 2020

      Question

      I'd like to know some information about sample preparation for your Ferritin Human ELISA Kits.

      I have to measure ferritin levels in human serum but I have to pre-treat my sera samples with a buffer.

      I have to completely set up the experiments so I have not decided yet in which buffer re-suspend my samples (i.e. phosphate, borate or acetate buffer…).

      Can I use your kits to measure sera sample previously re-suspended in this kind of buffers or they interfere with your ELISA assay? Are there other buffers that interfere (or not) with your ELISA assay?

      Do I have to re-suspend also standard samples (or dilute my sera sample) in the same buffer I used for pre-treatment?

      Read More
      Answer

      ab108698 Ferritin Human ELISA Kit: for pre-treatment of serum samples we recommend HEPES buffer.

      ab108837 Ferritin Human ELISA KIt: I am sorry this is not tested with other buffers, so we would not be able to advise which buffer to use. Therefore, we recommend to use the buffers that come with the kit in this case.

      Read More

      Question

      Daer abcam technical support team

      i want to know about

      c-Kit Human ELISA Kit (ab45924)
      Ferritin Human ELISA Kit (ab108698)

      abcam sent the kit by format 96 well plate or strip in 96 well plate.
      please advise me.

      Read More
      Answer


      The c-Kit Human ELISA Kit (ab45924) and Ferritin Human ELISA Kit (ab108698) both come with strip wells provided.

      Read More

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