Human Fas Ligand ELISA Kit (APTL) (ab100515)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 1.37 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Fas Ligand ELISA Kit (APTL)
Fas Ligand キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2 pg/ml -
検出範囲
1.37 pg/ml - 1000 pg/ml -
添加回収試験
75 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 76.99 70% - 90% Serum 76.36 70% - 87% Plasma 73.42 67% - 86% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Fas Ligand (APTL) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fas Ligand in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Fas Ligand 2 vials Fas Ligand Microplate (12 x 8 wells) 1 unit Recombinant Human Fas Ligand Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. -
関連疾患
Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
N-glycosylated.
The soluble form derives from the membrane form by proteolytic processing. -
細胞内局在
Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface. - Information by UniProt
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別名
- ALPS1B
- Apoptosis (APO 1) antigen ligand 1
- Apoptosis antigen ligand
see all -
参照データベース
- Entrez Gene: 356 Human
- Omim: 134638 Human
- SwissProt: P48023 Human
- Unigene: 2007 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab100515 は 2 報の論文で使用されています。
- Van den Broek B et al. Microglial derived extracellular vesicles activate autophagy and mediate multi-target signaling to maintain cellular homeostasis. J Extracell Vesicles 10:e12022 (2020). PubMed: 33708355
- Zhang Z et al. Astragalus mongholicus (Fisch.) Bge Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155. Front Pediatr 6:139 (2018). PubMed: 29977885