製品の概要

  • 製品名

    Human Factor XIII ELISA Kit
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8.7%
  • サンプルの種類

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    = 2.1 ng/ml
  • 検出範囲

    5 ng/ml - 80 ng/ml
  • 添加回収試験

    99 %

  • 全工程の試験時間

    4h 0m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Human
  • 製品の概要

    Abcam’s Factor XIII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of factor XIII in plasma, serum, milk, urine, saliva, and cell culture supernatants.


    A Factor XIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XIII captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    60X Biotinylated Human Factor XIII Antibody 1 x 100µl
    Chromogen Substrate 1 x 8ml
    Factor XIII Microplate (12 x 8 well strips) 1 unit
    Factor XIII Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究分野

  • 機能

    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
  • 関連疾患

    Factor XIII subunit A deficiency
  • 配列類似性

    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • 翻訳後修飾

    The activation peptide is released by thrombin.
  • 細胞内局在

    Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt
  • 別名

    • Coagulation factor XIII A chain
    • Coagulation factor XIIIa
    • F13A_HUMAN
    • F13a1
    • Protein-glutamine gamma-glutamyltransferase A chain
    • Transglutaminase A chain
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

画像

  • Representative Standard Curve using ab108836

プロトコール

参考文献

This product has been referenced in:

  • Peltier J  et al. Activation peptide of the coagulation factor XIII (AP-F13A1) as a new biomarker for the screening of colorectal cancer. Clin Proteomics 15:15 (2018). Read more (PubMed: 29657559) »
  • Liang Y  et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). Read more (PubMed: 29599912) »
See all 2 Publications for this product

レビューと Q&A

1-6 of 6 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

The kit using different antibodies, but the binding site is not mapped. Since they both polyclonal antibody, it may have multiple binding sites for protein.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for contacting us.


Yes, the kit will detect both the A and B forms.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thanks for your enquiry!

Please contact our bulk order specialist, Andrea Gray at mailto:Andrea.Gray@abcam.com, regarding discounts on bulk order purchases.

I hope this is helpful. Please contact me again if you have any further questions.

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Answer

Thank you for your call last week and for your patience while I've been in touch with the lab regarding your questions.

I've heard back from my contact at the lab, and she agrees that the OD values are lower than expected. An OD of 1.5-2.0 would be expected for the highest standard.She also believes this could have been caused by the breakduring the protocol, and she recommends repeating the experimentonce more without the 45 minute break, and compare the results.


It is also fine to optimize the dilution of the samples, and 1:1000 is only a suggested startingdilution. it would be a good idea to test various dilutions to find the optimal dilution factor for your plasma samples.

I hope this information will be useful. Please keep me updated with any progress, and let me know about the results after repeating the assay. If there is anything else that we can do for you, I will be happy to help you furhter.

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Answer

Thank you for your call the other day.

I am happy to confirm that the kit ab108836 against the human Factor XIII will recognize the full length protein as well as the fragment of the 37 first amino acids. I have just received the confirmation from the laboratory.

I have checked also for the antibodies against Factor XIII:There is none which is specifically targeted to this region. We have several polyclonal antibodies which are against the full length protein. They might recognize the fragment as well, we can however not be sure. Please let me know if you would like me to investigate those further.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

This ELISA kit will recognize factor XIIIa and factor XIII.  I hope this is helpful. Please contact me again if you have any further questions.  

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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