Human Factor XIII ELISA Kit (ab108836)
Key features and details
- Sensitivity: 1.5 ng/ml
- Range: 5 ng/ml - 80 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Factor XIII ELISA Kit
Factor XIIIa キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 9.4% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Tissue, Cell Lysate, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 1.5 ng/ml -
検出範囲
5 ng/ml - 80 ng/ml -
添加回収試験
99 %
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全工程の試験時間
4h 0m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Factor XIII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of factor XIII in plasma, serum, milk, urine, saliva, cell culture, and cell lysate samples.
A Factor XIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XIII captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor XIII Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Factor XIII Microplate (12 x 8 well strips) 1 unit Factor XIII Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. -
関連疾患
Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. -
配列類似性
Belongs to the transglutaminase superfamily. Transglutaminase family. -
翻訳後修飾
The activation peptide is released by thrombin. -
細胞内局在
Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma. - Information by UniProt
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別名
- bA525O21.1 (coagulation factor XIII, A1 polypeptide)
- Coagulation factor XIII A chain
- Coagulation factor XIII A1 polypeptide
see all -
参照データベース
- Entrez Gene: 2162 Human
- Omim: 134570 Human
- SwissProt: P00488 Human
- Unigene: 335513 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab108836 は 3 報の論文で使用されています。
- Matveeva VG et al. Advantages of Fibrin Polymerization Method without the Use of Exogenous Thrombin for Vascular Tissue Engineering Applications. Biomedicines 10:N/A (2022). PubMed: 35453539
- Peltier J et al. Activation peptide of the coagulation factor XIII (AP-F13A1) as a new biomarker for the screening of colorectal cancer. Clin Proteomics 15:15 (2018). PubMed: 29657559
- Liang Y et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). PubMed: 29599912