製品の概要

  • 製品名

    Human Factor X ELISA Kit
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • サンプルの種類

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    = 1.5 ng/ml
  • 検出範囲

    1.563 ng/ml - 100 ng/ml
  • 添加回収試験

    97.5 %

  • 全工程の試験時間

    4h 00m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Human
  • 製品の概要

    Abcam’s Factor X Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor X concentrations in plasma, serum, milk, urine, saliva and cell culture supernatants.


    A Factor X specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor X captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Factor X Microplate (12 x 8 well strips) 1 unit
    Factor X Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究分野

  • 機能

    Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
  • 組織特異性

    Plasma; synthesized in the liver.
  • 関連疾患

    Factor X deficiency
  • 配列類似性

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾

    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • Activated factor Xa heavy chain
    • F10
    • FA10_HUMAN
    • Stuart factor
    • Stuart-Prower factor
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

画像

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Factor X measured in undiluted culture supernatants with MCF7 levels below level of detection (duplicates +/- SD).

  • Factor X measured in biological fluids (duplicates +/- SD). Serum and plasma were tested at 1/300-1/3000; other samples at 1/1-1/30.

  • Representative Standard Curve using ab108832

プロトコール

参考文献

ab108832 has not yet been referenced specifically in any publications.

レビューと Q&A

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us and your interest in our products.

The Factor X Human ELISA kit, ab108832 enables both FX and FXa to detected in a range of biological samples.

I hope this information has been helpful. If you have any further questions, please do not hesitate to let me know.

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Answer

Thank you for alerting us to this mistake in the protocol.

I can confirm that the biotinylated antibody (page 10, step 5) should be incubated for 1 hour.

The protocol will be up-dated as soon as possible.

We wish you good luck with your experiments and a good day.

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Answer

Thank you for your inquiry.

ab108832 may be storedfor up tosix monthat 2 to 8°C or -20°C from the date of shipment.

Thiskits as all our products is covered by our Abpromiseguarantee for six month afterdate of purchase:

https://www.abcam.com/abpromise

Opened diluents may be stored for up to 1 month at 2 to 8°C.

Please store reconstituted reagents at -20°C or below. Opened unused strip wells may bereturned to the foil pouch with the desiccant pack, reseal along zip-seal and may be stored for up to 1 month in a vacuum desiccator.

I can confirm that the lead time is currently 1-2 weeks.

I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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