Human Factor VII ELISA Kit (with plasma controls) (ab168545)
Key features and details
- Sensitivity: 0.23 ng/ml
- Range: 2 ng/ml - 22.5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Factor VII ELISA Kit (with plasma controls)
Factor VII キット 製品一覧 -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.2% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 9.5% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
> 0.23 ng/ml -
検出範囲
2 ng/ml - 22.5 ng/ml -
添加回収試験
96 %
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全工程の試験時間
4h 00m -
種交差性
交差種: Human -
製品の概要
Abcam’s Factor VII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor VII levels in cell culture supernatants, serum and plasma.
A Factor VII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor VII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor VII captured in plate.
Get results in 90 minutes with Human Factor VII ELISA Kit (ab190810) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor VII Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Factor VII Microplate (12 x 8 well strips) 1 unit Factor VII Standard 1 vial Low control (Depleted Human Plasma) 1 vial Positive control (Reference Plasma Control) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. -
組織特異性
Plasma. -
関連疾患
Defects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. FA7D is a rare hereditary hemorrhagic disease. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Numerous subjects are completely asymptomatic despite a very low F7 level. -
配列類似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
翻訳後修飾
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Coagulation factor VII
- coagulation factor VII (serum prothrombin conversion accelerator)
- Eptacog alfa
see all -
参照データベース
- Entrez Gene: 2155 Human
- Omim: 613878 Human
- SwissProt: P08709 Human
- Unigene: 36989 Human
関連製品
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SimpleStep ELISA kits
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab168545 は 2 報の論文で使用されています。
- Cohen CT et al. Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts. Sci Rep 10:2005 (2020). PubMed: 32029851
- Wang H et al. N-Glycan-calnexin interactions in human factor VII secretion and deficiency. Int J Biochem Cell Biol 113:67-74 (2019). PubMed: 31185295