Human Factor V ELISA Kit (ab137976)
Key features and details
- Sensitivity: 49 pg/ml
- Range: 2.5 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Factor V ELISA Kit
Factor V キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 10.9% -
サンプルの種類
Cell culture supernatant, Milk, Urine, Plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
> 49 pg/ml -
検出範囲
2.5 ng/ml - 10 ng/ml -
添加回収試験
102 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Milk 91% - 103% Urine 94% - 102% Plasma 94% - 104% -
全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Factor V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor V in plasma, urine, milk, and cell culture supernatants.
A Factor V specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor V specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor V captured in plate.
Factor V Standard is a native protein.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor V Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Factor V Microplate (12 x 8 well strips) 1 unit Factor V Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. -
組織特異性
Plasma. -
関連疾患
Factor V deficiency
Thrombophilia due to activated protein C resistance
Budd-Chiari syndrome
Ischemic stroke
Pregnancy loss, recurrent, 1 -
配列類似性
Belongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 2 F5/8 type C domains.
Contains 6 plastocyanin-like domains. -
ドメイン
Domain B contains 35 x 9 AA tandem repeats, and 2 x 17 AA repeats. -
翻訳後修飾
Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).
Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.
Activated protein C inactivates factor V and factor Va by proteolytic degradation.
Phosphorylation sites are present in the extracellular medium. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Activated protein C cofactor
- APC cofactor
- Coagulation factor V
see all -
参照データベース
- Entrez Gene: 2153 Human
- Omim: 612309 Human
- SwissProt: P12259 Human
- Unigene: 30054 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab137976 は 2 報の論文で使用されています。
- Wang J et al. Coagulation factor V is a T-cell inhibitor expressed by leukocytes in COVID-19. iScience 25:103971 (2022). PubMed: 35224470
- Ward MP et al. Procoagulant activity in high grade serous ovarian cancer patients following neoadjuvant chemotherapy-The role of the activated protein C pathway. Thromb Res 200:91-98 (2021). PubMed: 33571724